Vitamin A (Beta-Carotene) Test
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Price
$125 per analysis
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Turnaround time 7 – 9 Business Days
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Rush Available
Technical data sheet
Print- Method description
Testing reports values for alpha, cis-beta, and trans-beta, total beta, and total carotene. Following enzymatic digestion designed for foods, the sample is homogenized with tetrahydrofuran in order to extract the carotenoids. The extract is filtered and quantitated using reverse phase HPLC with UV/Vis detection.
- Acceptable matrices
Food and Food Ingredients
- Unacceptable matrices
High fat samples (>50%), micro-encapsulated samples, high or low pH matrices (vinegar)
- Limit of quantitation
0.025 mcg/100g
- Equipment
HPLC-UV/VIS
- Method reference
Internally Developed Method
- Reportable unit
IU/100g, mcg RAE/100g. RAE = Retinol Activity Equivalents.
- Sample size requirements
15 g
- Information required by submitter
Please supply estimates
- Additional information
α and β-carotenes are plant pigments. This method measures the quantity of fortified and natural-occurring α and β-carotenes in food products and supplements. For products containing multiple forms of Vitamin A, carotenes and retinol can be added together to meet requirements for the NLEA nutrition label. Please let us know if results need to be reported as Dietary Carotenes (mcg RAE/100g) or Supplemental Carotenes (Supp. mcg RAE/100g) for your sample.
Common Vitamin A (Beta-Carotene) Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
We will report the Carotene results in mcg RAE (retinol activity equivalents) per 100g of sample. Upon request, we can also report in IU (international units). The mcg RAE unit is now the standard Vitamin A unit for nutrition labeling in the United States.
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