*Price of the test:
$98 per analysis
Acceptable Matrices:
This method is applicable to raw and processed foods, such as baby foods, vegetables, fruits, supplements, and juices.
Unacceptable Matrices:
Samples with >50% fat and samples that are micro-encapsulated.
Samples with high levels of salt may not give reliable results.
Detection Limit:
Any concentration greater than 0.025 mcg RAE/100g is detectable for dietary carotenes.
For supplemental carotenes, any concentration greater than 0.3 Supplemental mcg RAE/100g is detectable for alpha, cis beta, Total beta carotene and Total carotene. For trans beta carotene, any concentration greater than 0.15 Supplemental mcg RAE/100g is detectable.
Sample Size Requirements:
10 grams of sample is needed. Samples should be kept frozen until analysis.
Equipment:
HPLC-UV/VIS
Reportable Units:
mcg RAE/100g
RAE = Retinol Activity Equivalents
Turnaround Time:
7-9 business days
Is rush testing available?
Yes
Method Reference:
Internally Developed Method
Method Description:
Testing reports values for alpha, cis-beta, and trans-beta, total beta, and total carotene. Following enzymatic digestion designed for foods, the sample is homogenized with tetrahydrofuran in order to extract the carotenoids. The extract is filtered and quantitated using reverse phase HPLC with UV/Vis detection.
Additional Information:
α and β-carotenes are plant pigments. This method measures the quantity of fortified and natural-occurring α and β-carotenes in food products and supplements. For products containing multiple forms of Vitamin A, carotenes and retinol can be added together to meet requirements for the NLEA nutrition label.
Please let us know if results need to be reported as Dietary Carotenes (mcg RAE/100g) or Supplemental Carotenes (Supp. mcg RAE/100g) for your sample.
Contact Us:
If you have questions about our testing services, please contact us at +1 (800) 245-5615.
*Prices and offerings are subject to change without notice.