Home / Sugar Profile by HPLC Test

Sugar Profile by HPLC Test

  • 113 per analysis
  • |
  • 7-9 Business Days
  • |
  • Rush Available
Our commitment to quality

You can expect high-quality, reliable results time after time.

Learn More
Sugar Profile by HPLC Test

Technical data sheet


Method description

This method directly determines the amounts of fructose, galactose, glucose, sucrose, maltose, and lactose. The sugars are extracted and cleaned-up; thereafter, the appropriate dilution is quantitated using High-Performance Liquid Chromatography (HPLC).

Acceptable matrices

Most raw and processed foods or ingredients.

Unacceptable matrices

See “Additional Information” section for samples containing sugar alcohols.

Samples containing stevia may experience interference.

Limit of quantitation

The detection level is 0.1%.


High-Performance Liquid Chromatography – RI detection

Method reference

AOAC 977.20

High-Pressure Liquid Chromatographic Determination of Carbohydrates in Food Products: Evaluation of Method, J.W. DeVries, J.C. Heroff, and D.C.Egberg, J. Assoc. Off. Anal. Chem. 62 p1292 (1979)

Reportable unit


Sample size requirements

20 grams

Information required by submitter

Please supply an estimate of the sugar and salt levels, if available. Please let us know if your sample contains any added or naturally occurring sugar alcohols.

Additional information

Sugar alcohols co-elute/interfere with simple sugars (fructose, galactose, glucose, sucrose, maltose, lactose). If your sample contains sugar alcohols and you need to determine total sugar, Sugar Alcohol Screen Test (Gas Chromatograph) will need to be requested in addition to this test. The simple sugar(s) experiencing interference will be reported using the Sugar Alcohol Screen Test.

Total Sugars will not be listed on the Final Report for samples containing sugar alcohols. To calculate total sugar, the simple sugars (fructose, galactose, glucose, sucrose, maltose, and lactose) reported from this method and the Sugar Alcohol Screen Test will need to be added manually upon receipt of the Final Report.

*Prices and offerings are subject to change without notice.

Common Sugar Profile by HPLC Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

Do you have a more specific question?

Our food testing experts are here to help.

"*" indicates required fields

This field is for validation purposes and should be left unchanged.
Ask a food testing expert a question

Let’s Get to Work!

Submit your order online and ship your samples today. If you have questions, we are always here to help.

Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.