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Sugar Profile by HPLC Test

  • Price

    $130 per analysis

  • Turnaround time

    7 - 9 Business Days

  • Rush


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Sugar Profile by HPLC Test

Technical data sheet


Method description

This method directly determines the amounts of fructose, galactose, glucose, sucrose, maltose, and lactose. The sugars are extracted and cleaned-up; thereafter, the appropriate dilution is quantitated using High-Performance Liquid Chromatography (HPLC).

Acceptable matrices

Food and Food Ingredients

Unacceptable matrices


Limit of quantitation



HPLC with an RI Detector

Method reference

AOAC 977.20 High-Pressure Liquid Chromatographic Determination of Carbohydrates in Food Products: Evaluation of Method, J.W. DeVries, J.C. Heroff, and D.C.Egberg, J. Assoc. Off. Anal. Chem. 62 p1292 (1979)

Reportable unit


Sample size requirements

60 g

Information required by submitter

Please supply estimates

Additional information

Sugar alcohols co-elute/interfere with simple sugars (fructose, galactose, glucose, sucrose, maltose, lactose). If your sample contains sugar alcohols and you need to determine total sugar, Sugar Alcohol Screen Test (Gas Chromatograph) will need to be requested in addition to this test. The simple sugar(s) experiencing interference will be reported using the Sugar Alcohol Screen Test. Total Sugars will not be listed on the Final Report for samples containing sugar alcohols. To calculate total sugar, the simple sugars (fructose, galactose, glucose, sucrose, maltose, and lactose) reported from this method and the Sugar Alcohol Screen Test will need to be added manually upon receipt of the Final Report.

*Prices and offerings are subject to change without notice.

Common Sugar Profile by HPLC Test Testing Questions

The sugar profile by HPLC (high-performance liquid chromatography) test is a chromatographic analysis that quantifies the amount of fructose, galactose, glucose, sucrose, maltose, and lactose in food products and raw ingredients.

Sugars are extracted from samples using solvent and then injected on an HPLC and analyzed against known standards.

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.