Fiber (Total - Rapid Integrated Method) Test
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478 per analysis | -
12 - 15 Business Days | -
Rush Available
Technical data sheet
PrintMethod description
Samples (defatted, if necessary) are digested four hours in a manner that simulates the human digestive system with three enzymes (pancreatic alpha-amylase, protease, and amyloglucosidase) to remove starch and protein. Ethanol is added to precipitate high molecular weight soluble dietary fiber. The resulting solution is filtered, and the residue is dried and weighed. Protein and ash are determined on the residue. The filtrate is captured, concentrated, and analyzed via HPLC to determine the value of low molecular weight soluble dietary fiber (LMWSF).
Total dietary fiber (TDF) is calculated as the weight of the residue minus protein and ash plus the low molecular weight soluble fiber determined by HPLC analysis.
Acceptable matrices
Foods and food ingredients
Unacceptable matrices
Stevia can cause interference with the chromatography. Samples containing >2% stevia will damage the instrument therefore cannot be run through this method. Glucomannan
Limit of quantitation
0.1%
Equipment
HPLC
Method reference
AOAC 2017.16 with modifications
Reportable unit
% (g/100g)
Sample size requirements
40 grams
Information required by submitter
Please supply expected estimates. Indicate matrix type and indicate if samples are high fat (>5%). Indicate whether the sample contains fructans (inulin or fructooligosaccarides (FOS)) or psyllium, flax and gum (i.e. guar gum).
Additional information
Measures total dietary fiber, including resistant starch and low molecular weight non-digestible oligosaccharides of DP =3. If a breakout of soluble and insoluble fiber as well as total dietary fiber is desired, please choose our Total, Soluble, and Insoluble Dietary Fiber Rapid Integrated method.
Please include notification for samples containing DP2 and DP3 carbohydrates (Isomalto-oligosaccharides (IMO), Maltiltol, Lactose).
Dietary Fiber is the portion of the carbohydrates not digested in the human gut. It is composed of both soluble and insoluble components. High-fat (>5%) samples require special handling.
Common Fiber (Total - Rapid Integrated Method) Test Testing Questions
While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.
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