Technical data sheet
Samples (defatted, if necessary) are digested four hours in a manner that simulates the human digestive system with three enzymes (pancreatic alpha-amylase, protease, and amyloglucosidase) to remove starch and protein. Ethanol is added to precipitate high molecular weight soluble dietary fiber. The resulting solution is filtered, and the residue is dried and weighed. Protein and ash are determined on the residue. The filtrate is captured, concentrated, and analyzed via HPLC to determine the value of low molecular weight soluble dietary fiber (LMWSF).
Total dietary fiber (TDF) is calculated as the weight of the residue minus protein and ash plus the low molecular weight soluble fiber determined by HPLC analysis.
Foods and food ingredients
Stevia can cause interference with the chromatography. Samples containing >2% stevia will damage the instrument therefore cannot be run through this method. Glucomannan
Limit of quantitation
AOAC 2017.16 with modifications
Sample size requirements
Information required by submitter
Please supply expected estimates. Indicate matrix type and indicate if samples are high fat (>5%). Indicate whether the sample contains fructans (inulin or fructooligosaccarides (FOS)) or psyllium, flax and gum (i.e. guar gum).
Measures total dietary fiber, including resistant starch and low molecular weight non-digestible oligosaccharides of DP =3. If a breakout of soluble and insoluble fiber as well as total dietary fiber is desired, please choose our Total, Soluble, and Insoluble Dietary Fiber Rapid Integrated method.
Please include notification for samples containing DP2 and DP3 carbohydrates (Isomalto-oligosaccharides (IMO), Maltiltol, Lactose).
Dietary Fiber is the portion of the carbohydrates not digested in the human gut. It is composed of both soluble and insoluble components. High-fat (>5%) samples require special handling.