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Fiber (Insoluble, Soluble, Total - Rapid Integrated Method) Test

  • Price

    $560 per analysis

  • Turnaround time

    12 - 15 Business Days

  • Rush

    Available

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Fiber (Insoluble, Soluble, Total - Rapid Integrated Method) Test

Technical data sheet

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Method description

Samples (defatted, if necessary) are digested with a mammalian alpha-amylase and an amyloglucosidase for 4 hours at 37°C to simulate the human digestive system. Following digestion, the sample is heated to 95°C to denature all enzymes and proteins present and digested at 60°C with protease. Insoluble digestion resistant material (dietary fiber) is filtered away and the amount of Insoluble Dietary Fiber (IDF) is determined gravimetrically with protein and ash content subtracted. Protein and ash are determined on the residue. The dietary fiber remaining in solution following the filtration is precipitated with ethanol and is collected in a second filtration step. The amount of dietary fiber collected in the filter is determined gravimetrically with protein and ash subtracted as above and is recorded as the Gravimetric Soluble Dietary Fiber (G-SDF). The filtrate containing the soluble dietary fiber not precipitated by the ethanol is concentrated and analyzed by High Performance Liquid Chromatography (HPLC) to determine the total HPLC-Soluble Dietary Fiber (HPLC-SDF) with a degree of polymerization (DP) greater than or equal to 3 (DP3). The total Soluble Dietary Fiber is calculated as the sum of the Gravimetric-Soluble Dietary Fiber and the HPLC-Soluble Dietary Fiber (SDF = G-SDF + HPLC-SDF). Total dietary fiber is calculated as the sum of the Insoluble Dietary Fiber and the Soluble Dietary Fiber (TDF = IDF + SDF).

Acceptable matrices

Foods and food ingredients

Unacceptable matrices

Samples containing more than 2% stevia

Limit of quantitation

Total Dietary Fiber - 0.2% Insoluble Fiber – 0.1% Soluble Dietary Fiber-0.2%

Equipment

HPLC

Method reference

AOAC 2022.01 with modifications

Reportable unit

% (g/100g)

Sample size requirements

25 g

Information required by submitter

Please supply estimates

Additional information

Please include notification for samples containing DP2 and DP3 carbohydrates (Isomalto-oligosaccharides (IMO), Maltiltol, Lactose). This test measures dietary fiber including resistant starch and non-digestible oligosaccharides of DP >3. Dietary Fiber is the portion of the carbohydrates not digested in the human gut. It is composed of both soluble and insoluble components. These methods are applicable to all food products; however, samples containing psyllium or fructans should be submitted under specific test codes. High-fat (>5%) samples require special handling.

*Prices and offerings are subject to change without notice.

Is this the most suitable fiber test for evaluating my product?

Analysis of Fiber Sources

Find your fiber source(s) on the chart and use the alpha reference to see how it aligns with each method. If the alpha reference is fully inside the method circle, that method is optimal for your fiber source(s). If it's outside or touching the circle's edge, the method won't fully capture the fiber. Remember to check applicable labeling regulations for allowed fiber sources.

  • Icon Logo Cellulose | Beta-Glucan | Psyllium | Gums | Lignin
  • Icon Logo Galacto-oligosaccharides-Raffinose(GOS)/Stachyose
  • Icon Logo Galactomannan | Arabinoxylan | Glucomannan
  • Icon Logo Resistant Starch RS4
  • Icon Logo Resistant Starch RS2 & RS3
  • Icon Logo Pectin | Alginates | Arabinogalactin | Isomalto-oligosaccharide (IMO)
  • Icon Logo Polydextrose | Fibersol®-2 | Resistant Maltodextrins | Soluble Corn Fiber (SCF)
  • Icon Logo Fructans (Inulin, FOS)
AOAC Method 2001.03 with AOAC Method 991.43
  • Icon Logo Cellulose | Beta-Glucan | Psyllium | Gums | Lignin
  • Icon Logo Galacto-oligosaccharides-Raffinose(GOS)/Stachyose
  • Icon Logo Galactomannan | Arabinoxylan | Glucomannan
  • Icon Logo Resistant Starch RS4
  • Icon Logo Resistant Starch RS2 & RS3
  • Icon Logo Pectin | Alginates | Arabinogalactin | Isomalto-oligosaccharide (IMO)
  • Icon Logo Polydextrose | Fibersol®-2 | Resistant Maltodextrins | Soluble Corn Fiber (SCF)
  • Icon Logo Fructans (Inulin, FOS)
AOAC Method 2017.16 AOAC Method 2022.01
  • Icon Logo Cellulose | Beta-Glucan | Psyllium | Gums | Lignin
  • Icon Logo Galacto-oligosaccharides-Raffinose(GOS)/Stachyose
  • Icon Logo Galactomannan | Arabinoxylan | Glucomannan
  • Icon Logo Resistant Starch RS4
  • Icon Logo Resistant Starch RS2 & RS3
  • Icon Logo Pectin | Alginates | Arabinogalactin | Isomalto-oligosaccharide (IMO)
  • Icon Logo Polydextrose | Fibersol®-2 | Resistant Maltodextrins | Soluble Corn Fiber (SCF)
  • Icon Logo Fructans (Inulin, FOS)
AOAC Method 985.29 AOAC Method 991.43
  • Icon Logo Cellulose | Beta-Glucan | Psyllium | Gums | Lignin
  • Icon Logo Galacto-oligosaccharides-Raffinose(GOS)/Stachyose
  • Icon Logo Galactomannan | Arabinoxylan | Glucomannan
  • Icon Logo Resistant Starch RS4
  • Icon Logo Resistant Starch RS2 & RS3
  • Icon Logo Pectin | Alginates | Arabinogalactin | Isomalto-oligosaccharide (IMO)
  • Icon Logo Polydextrose | Fibersol®-2 | Resistant Maltodextrins | Soluble Corn Fiber (SCF)
  • Icon Logo Fructans (Inulin, FOS)
AOAC Method 2009.01 AOAC Method 2011.25
Common Fiber (Insoluble, Soluble, Total - Rapid Integrated Method) Test Testing Questions

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

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