*Price of the test:
$268 per analysis
Unacceptable Matrices:
High-fat matrices (i.e. fish oils), popcorn, concentrates
Quantitation Limit:
0.500 mcg/100g – 250 mcg/100g
Sample Size Requirements:
100 grams for dairy products
30 grams for all other samples
Equipment:
LC-MS/MS
Reportable Units:
mcg/100g
Turnaround Time:
7-9 business days
Is rush testing available?
Yes
Method Reference:
AOAC 995.05, 992.26, 2002.05 with modifications, Journal of AOAC Int vol 95, No. 3, 2012
Method Description:
This method determines the amount of Vitamin D3 (cholecalciferol) in various food products.
An internal standard is added, and the sample is saponified in ethanolic KOH. It is then extracted into a heptane organic phase and evaporated to dryness. The sample extract is reconstituted and fractions of the internal standard and D3 are collected using a solid phase extraction (SPE) column. The fractions are then concentrated, and the level of D3 is determined using LC-MS/MS.
Information Required by Submitter:
Please supply estimates in mcg/100g.
Additional Information:
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.
Please let us know if your samples contain Vitamin D2 and we will discuss testing options.
Contact Us:
If you have questions about our testing services, please contact us at +1 (800) 245-5615.
*Prices and offerings are subject to change without notice.