Technical data sheet
This method determines the amount of Vitamin D3 (cholecalciferol) in various food products.
An internal standard is added, and the sample is saponified in ethanolic KOH. It is then extracted into a heptane organic phase and evaporated to dryness. The sample extract is reconstituted and fractions of the internal standard and D3 are collected using a solid phase extraction (SPE) column. The fractions are then concentrated, and the level of D3 is determined using LC-MS/MS.
High-fat matrices (i.e. fish oils), popcorn, concentrates
Limit of quantitation
0.500 mcg/100g – 250 mcg/100g
AOAC 995.05, 992.26, 2002.05 with modifications, Journal of AOAC Int vol 95, No. 3, 2012
Sample size requirements
50 grams for dairy products
10 grams for all other samples
Information required by submitter
Please supply estimates in mcg/100g.
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.
Please let us know if your samples contain Vitamin D2 and we will discuss testing options.