Vitamin D Test
- Price
$300 per analysis
- Turnaround time
7 - 9 Business Days
- Rush
Available
Technical data sheet
- Method description
This method determines the amount of Vitamin D3 (cholecalciferol) in various food products. An internal standard is added, and the sample is saponified in ethanolic KOH. It is then extracted into a heptane organic phase and evaporated to dryness. The sample extract is reconstituted and fractions of the internal standard and D3 are collected using a solid phase extraction (SPE) column. The fractions are then concentrated, and the level of D3 is determined using LC-MS/MS.
- Acceptable matrices
Food and Food Ingredients
- Unacceptable matrices
Concentrates and premixes
- Limit of quantitation
0.500 mcg/100g - 250 mcg/100g
- Equipment
LC-MS/MS
- Method reference
AOAC 995.05, 992.26, 2002.05 with modifications, Journal of AOAC Int vol 95, No. 3, 2012
- Reportable unit
IU/100g, mcg/100g
- Sample size requirements
60 g
- Information required by submitter
Please supply estimates
- Additional information
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. Please let us know if your samples contain Vitamin D2 and we will discuss testing options.
Common Vitamin D Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
Our food testing experts are here to help.
"*" indicates required fields
Helpful content related to Vitamin D Test
Submit your order online and ship your samples today. If you have questions, we are always here to help.
A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.