Skip to main content

Home / Vitamin D Test

Vitamin D Test

  • Price

    $300 per analysis

  • Turnaround time

    7 - 9 Business Days

  • Rush


Our commitment to quality

You can expect high-quality, reliable results time after time.

Learn More
Vitamin D Test

Technical data sheet


Method description

This method determines the amount of Vitamin D3 (cholecalciferol) in various food products. An internal standard is added, and the sample is saponified in ethanolic KOH. It is then extracted into a heptane organic phase and evaporated to dryness. The sample extract is reconstituted and fractions of the internal standard and D3 are collected using a solid phase extraction (SPE) column. The fractions are then concentrated, and the level of D3 is determined using LC-MS/MS.

Acceptable matrices

Food and Food Ingredients

Unacceptable matrices

Concentrates and premixes

Limit of quantitation

0.500 mcg/100g - 250 mcg/100g



Method reference

AOAC 995.05, 992.26, 2002.05 with modifications, Journal of AOAC Int vol 95, No. 3, 2012

Reportable unit

IU/100g, mcg/100g

Sample size requirements

60 g

Information required by submitter

Please supply estimates

Additional information

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. Please let us know if your samples contain Vitamin D2 and we will discuss testing options.

*Prices and offerings are subject to change without notice.

Common Vitamin D Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

Do you have a more specific question?

Our food testing experts are here to help.

"*" indicates required fields

This field is for validation purposes and should be left unchanged.
Ask a food testing expert a question

Let’s Get to Work!

Submit your order online and ship your samples today. If you have questions, we are always here to help.

Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.