Price of the test:
$268 per analysis
Unacceptable Matrices:
High fat matrices (i.e. fish oils), popcorn
Limit of Quantitation:
40 IU/100g
Sample Size Requirements:
100 grams for dairy products
30 grams for all other samples
Equipment:
High Performance Liquid Chromatography –UV/VIS detector
HPLC-MS (used for verification of analyte presence, as needed)
Reportable Units:
IU/100g
Turn Around Time:
7-9 Business Days
Is Rush Available?
Yes
Method Reference:
AOAC 2002.05 with modifications
Method Description:
An internal standard is added and the sample is saponified in ethanolic KOH. It is then extracted into a heptane organic phase, and evaporated to dryness. The sample extract is reconstituted and fractions of the internal standard, D2 and D3 are collected using a semi-preparative normal phase HPLC column. The fractions are then concentrated, and the levels of D2 and D3 are determined using reverse-phase HPLC with UV detection.
Information Required by Submitter:
Please supply estimates in IU/100g
Additional Information:
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.