Price of the test:
$107 per analysis
Acceptable Matrices:
This method is applicable to cereals, beverages and dairy products containing added VB6 and/or other free forms or vitamin B6.
Unacceptable Matrices:
This method is not designed for use on vitamin concentrates.
Limit of Quantitation:
0.002 mg/100g to 4000 mg/100 g
Sample Size Requirements:
10 grams
Equipment:
Automated pipettor system
Automated plate reader system
Reportable Units:
mg/100g
Turn Around Time:
7-9 Business Days
Is Rush Available?
Yes
Method Reference:
AOAC 961.15, 985.32, 960.46
Method Description:
This is a microbiological assay employing the organism Saccharomyces uvarum to determine the quantity of Vitamin B6 (pyridoxine, pyridoxal & pyridoxpyridoxine in food products and vitamin concentrates.
The ground, homogenized sample is dissolved or dispersed in an acidic extraction solution. The mixture is autoclaved at 121 – 123 °C to extract vitamin B6. The pH of the extract is adjusted to 4.4 and diluted with a basal medium containing all required growth nutrients for the microorganism except VB6. The mixture is inoculated with the microorganism and then incubated for 22 hours at 30°C. The growth response of the microorganism is compared quantitatively to that of known standard solutions of VB6.
The result is expressed as vitamin B6 (pyridoxine). The method is semi-automated utilizing an Automated Pipettor System and an Automated Plate Reader System.
Information Required by Submitter:
Please supply an estimate with your sample.
Additional Information:
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.
This method is not designed for use on vitamin concentrates.