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Vitamin B6 (Pyridoxine) Test

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Vitamin B6 (Pyridoxine) Test
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Technical data sheet

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Method description

This is a microbiological assay employing the organism Saccharomyces uvarum to determine the quantity of vitamin B6 (pyridoxine, pyridoxal, and pyridoxpyridoxine) in food products and vitamin concentrates.

The ground, homogenized sample is dissolved or dispersed in an acidic extraction solution. The mixture is autoclaved at 121 – 123 °C to extract vitamin B6. The pH of the extract is adjusted to 4.4 and diluted with a basal medium containing all required growth nutrients for the microorganism except vitamin B6. The mixture is inoculated with the microorganism and incubated for 22 hours at 30°C. The growth response of the microorganism is compared quantitatively to that of known standard solutions of vitamin B6.

The result is expressed as vitamin B6 (pyridoxine). The method is semi-automated utilizing an automated pipettor system and an automated plate reader system.

Acceptable matrices

This method is applicable to cereals, beverages, and dairy products containing added vitamin B6 and/or other free forms of vitamin B6.

Unacceptable matrices

This method is not designed for use on vitamin concentrates.

Limit of quantitation

0.002 mg/100g to 4000 mg/100 g

Equipment

Automated pipettor system
Automated plate reader system

Method reference

AOAC 961.15, 985.32, 960.46

Reportable unit

mg/100g

Sample size requirements

10 grams

Information required by submitter

Please supply an estimate with your sample.

Additional information

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.

This method is not designed for use on vitamin concentrates.

*Prices and offerings are subject to change without notice.

Common Vitamin B6 (Pyridoxine) Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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