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Vitamin B5 (Pantothenic) Test

  • Price

    $130 per analysis

  • Turnaround time

    7 - 9 Business Days

  • Rush


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Vitamin B5 (Pantothenic) Test

Technical data sheet


Method description

This is a microbiological method employing the organism Lactobacillus plantarum to determine calcium pantothenate or other free forms of pantothenic acid in vitamin concentrates and food products. The ground, homogenized sample is dissolved or dispersed in double-distilled water. The pH is adjusted to 5.65 and the mixture is autoclaved at 121 - 123°C to extract pantothenate or other free forms of pantothenic acid. The extract is diluted with a basal medium containing all required growth nutrients for Lactobacillus plantarum except pantothenate. The mixture is then inoculated with the microorganism and incubated at 37°C for 22 hours. The growth response of the microorganism is compared quantitatively to that of known standard solutions of calcium pantothenate. The method is semi-automated, utilizing an automated pipettor system and an automated plate reader system.

Acceptable matrices

Cereal, vitamin concentrates, fruit juices, nutritional supplements, and meal replacement beverages containing added calcium pantothenate and/or other free forms of pantothenic acid.

Unacceptable matrices

High fat dairy products/powders, infant formula, premixes/concentrates/supplements above 40%

Limit of quantitation

0.02 - 40,000 mg/100g


Automated pipettor system Automated plate reader system

Method reference

AOAC 945.74, AOAC 960.46, AOAC 992.07

Reportable unit


Sample size requirements

10 g

Information required by submitter

Please supply estimates

Additional information

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. This method is not designed for use on vitamin concentrates.

*Prices and offerings are subject to change without notice.

Common Vitamin B5 (Pantothenic) Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.