Price of the test:
$72 per analysis
Acceptable Matrices:
This method is applicable to cereals, beverages and dairy products containing naturally occurring and / or added niacin and niacinamide.
Unacceptable Matrices:
This method is not designed for use on vitamin concentrates.
Limit of Quantitation:
0.007mg / 100g to 20,000mg/100g
Sample Size Requirements:
10 grams
Equipment:
Automated pipettor system
Automated plate reader system
Reportable Units:
mg/100g
Turn Around Time:
7-9 Business Days
Is Rush Available?
Yes
Method Reference:
AOAC 944.13
AOAC 960.46
Microbiological Methods
Method Description:
This is a microbiological method employing the organism Lactobacillus plantarum to determine niacin in food and vitamin concentrates.
The ground, homogenous sample is dissolved in a 1N H2SO4 solution and autoclaved for 30 minutes at 121 – 123 °C. The pH of the solution is adjusted to 6.8. The diluted extract is added to a basal medium containing all required growth nutrients for the microorganism except niacin. The mixture is inoculated with the microorganism and then incubated for 22 hours at 37°C. The growth response of the microorganism is compared quantitatively to that of known standard solutions of niacin. The method is semi-automated utilizing an Automated Pipettor System and an Automated Plate Reader System.
Information Required by Submitter:
Please supply an estimate with your sample.
Additional Information:
This test is ISO/IEC 17025 accredited by A2LA, Certificate # 2769.01.
This method is not designed for use on vitamin concentrates.