Vitamin B3 (Niacin) Test
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Price
$95 per analysis
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Turnaround time 7 – 9 Business Days
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Rush Available
Technical data sheet
Print- Method description
This is a microbiological method employing the organism Lactobacillus plantarum to determine niacin in food. The ground, homogenous sample is dissolved in a 1N H2SO4 solution and autoclaved for 30 minutes at 121 - 123 °C. The pH of the solution is adjusted to 6.8. The diluted extract is added to a basal medium containing all required growth nutrients for the microorganism except niacin. The mixture is inoculated with the microorganism and then incubated for 22 hours at 37°C. The growth response of the microorganism is compared quantitatively to that of known standard solutions of niacin. The method is semi-automated utilizing an automated pipettor system and an automated plate reader system.
- Acceptable matrices
The method is applicable to cereals, beverages, vitamin concentrates and dairy products containing naturally occurring and/or added niacin and niacinamide.
- Unacceptable matrices
premixes, supplements, concentrates above 20%
- Limit of quantitation
0.05mg/100g to 20,000mg/100g
- Equipment
Automated pipettor system Automated plate reader system
- Method reference
AOAC 944.13 AOAC 960.46 Microbiological methods
- Reportable unit
mg/100g
- Sample size requirements
10 g
- Information required by submitter
Please supply estimates
- Additional information
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. This method is not designed for use on vitamin concentrates.
Common Vitamin B3 (Niacin) Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
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