Technical data sheet
Samples are autoclaved in HCl to breakdown the matrix. Potassium permanganate is added to the filtrate to oxidize interfering fluorescent compounds. Sodium bisulfite is then added to decolorize residual potassium permanganate. The fluorescence intensity (in the 440-550 nm range) of the vitamin is then measured. Blank measurements to allow for interfering fluorescence are performed on samples where sodium hydrosulfite is added that quenches riboflavin’s fluorescence. The net fluorescence is compared to a prepared standard curve.
Auto-analyzer with fluorescence detection
AOAC 942.23, 970.65 and 981.15
Sample size requirements
10 grams for dry samples
20 grams for liquid samples
Information required by submitter
Please supply an estimate of expected riboflavin content and indicate the presence of iron in samples.
Samples of vitamin concentrates should be clearly labeled.
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.
High iron content can interfere with the reaction chemistry. Please indicate the presence of high levels of iron.
Vitamin concentrate results typically have higher-than-normal method variability.