*Price of the test:
$75 per analysis
0.02 mg/100g to 0.1 mg/100g, depending on sample weight
Sample Size Requirements:
10 grams for dry samples
20 grams for liquid samples
Auto-analyzer with fluorescence detection
7-9 business days
Is rush testing available?
AOAC 942.23, 970.65 and 981.15
Samples are autoclaved in HCl to breakdown the matrix. Potassium permanganate is added to the filtrate to oxidize interfering fluorescent compounds. Sodium bisulfite is then added to decolorize residual potassium permanganate. The fluorescence intensity (in the 440-550 nm range) of the vitamin is then measured. Blank measurements to allow for interfering fluorescence are performed on samples where sodium hydrosulfite is added that quenches riboflavin’s fluorescence. The net fluorescence is compared to a prepared standard curve.
Information Required by Submitter:
Please supply an estimate of expected riboflavin content and indicate the presence of iron in samples.
Samples of vitamin concentrates should be clearly labeled.
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.
High iron content can interfere with the reaction chemistry. Please indicate the presence of high levels of iron.
Vitamin concentrate results typically have higher-than-normal method variability.
If you have questions about our testing services, please contact us at +1 (800) 245-5615.
*Prices and offerings are subject to change without notice.