Price of the test:
$85 per analysis
Limit of Detection:
0.02 mg/100g to 0.1 mg/100g (depending on sample weight)
Sample Size Requirements:
10 grams for dry samples
20 grams for liquid samples
Auto-analyzer with fluorescence detection
Turn Around Time:
7-9 Business Days
Is Rush Available?
AOAC 942.23, 970.65 and 981.15
Samples are extracted by autoclaving in HCl to breakdown the matrix. A buffer and enzyme are added to convert any thiamin phosphate to thiamin. Alkaline potassium ferricyanide is added to the filtrates to oxidize and convert thiamin to thiochrome. The thiochrome is extracted into iso-butyl alcohol and measured fluorometrically. Blanks are determined by adding NaOH instead of ferricyanide. The thiamin level is determined by comparing the net fluorescence to a standard curve.
Information Required by Submitter:
Please supply an estimate of expected thiamine content.
Please indicate the presence of high levels of iron.
Samples of “Vitamin Concentrates” should be clearly labeled.
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.
High iron content may interfere with the reaction chemistry. Vitamin concentrate results typically have higher-than-normal method variability.