Vitamin B1 (Thiamin) Test
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Price
$95 per analysis
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Turnaround time 7 - 9 Business Days
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Rush Available
Technical data sheet
Print- Method description
Samples are extracted by autoclaving in HCl to breakdown the matrix. A buffer and enzyme are added to convert any thiamin phosphate to thiamin. Alkaline potassium ferricyanide is added to the filtrates to oxidize and convert thiamin to thiochrome. The thiochrome is extracted into iso-butyl alcohol and measured fluorometrically. Blanks are determined by adding NaOH instead of ferricyanide. The thiamin level is determined by comparing the net fluorescence to a standard curve.
- Acceptable matrices
Food and Food Ingredients
- Limit of quantitation
0.02 mg/100g to 0.1 mg/100g, depending on sample weight
- Equipment
Fluorescence Spectrophotometer, Auto-analyzer with fluorescence detection
- Method reference
AOAC 942.23, 970.65 and 981.15
- Reportable unit
mg/100g
- Sample size requirements
10 g
- Information required by submitter
Please supply an estimate of expected thiamine content and indicate the presence of high levels of iron. Samples of vitamin Concentrates should be clearly labeled.
- Additional information
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.
Common Vitamin B1 (Thiamin) Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
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