Technical data sheet
Sugar Profile: This method directly determines the amounts of simple sugars (fructose, galactose, glucose, sucrose, maltose, and lactose). The sugars are extracted and cleaned-up; thereafter, the appropriate dilution is quantitated using High-Performance Liquid Chromatography (HPLC).
Sugar Alcohol Screen: This method determines the amounts of Sorbitol, Maltitol, Erythritol, Xylitol, Mannitol, Isomalt, and Lactitol. Samples are extracted, components are converted to oximes, derivatized, and analyzed using gas chromatography. Calculations are based on response factors of known standards.
Most raw and processed foods or ingredients.
Limit of quantitation
The detection level is 0.1%.
Sugar Profile: High-Performance Liquid Chromatography – RI detection
Sugar Alcohol Screen: Gas chromatograph with a flame ionization detector
Sugar Profile: AOAC 977.20, High-Pressure Liquid Chromatographic Determination of Carbohydrates in Food Products: Evaluation of Method, J.W. DeVries, J.C. Heroff, and D.C.Egberg, J. Assoc. Off. Anal. Chem. 62 p1292 (1979)
Sugar Alcohol Screen: Internally-developed method.
Sample size requirements
Information required by submitter
Please supply an estimate of the sugar and salt levels, if available.
Sugar alcohols co-elute/interfere with simple sugars during the analysis of the Sugar Profile, resulting in the need to run both Sugar Profile by HPLC Test and Sugar Alcohol Screen (Gas Chromatography) in order to determine total sugar in samples that contain added or naturally occurring sugar alcohols.
The simple sugar(s) experiencing interference during the Sugar Profile Test will be reported under the Sugar Alcohol Screen.
Total Sugars will be reported as a single line item on the Final Report, listed as “Sugars, Total by Calculation” which is a sum of all reportable simple sugars (fructose, galactose, glucose, sucrose, maltose, lactose) across both the Sugar Profile Test and the Sugar Alcohol Screen.