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Sugars, Total by Calculation Test

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Sugars, Total by Calculation Test

Technical data sheet


Method description

Sugar Profile: This method directly determines the amounts of simple sugars (fructose, galactose, glucose, sucrose, maltose, and lactose). The sugars are extracted and cleaned-up; thereafter, the appropriate dilution is quantitated using High-Performance Liquid Chromatography (HPLC).

Sugar Alcohol Screen: This method determines the amounts of Sorbitol, Maltitol, Erythritol, Xylitol, Mannitol, Isomalt, and Lactitol. Samples are extracted, components are converted to oximes, derivatized, and analyzed using gas chromatography. Calculations are based on response factors of known standards.

Acceptable matrices

Most raw and processed foods or ingredients.

Limit of quantitation

The detection level is 0.1%.


Sugar Profile: High-Performance Liquid Chromatography – RI detection

Sugar Alcohol Screen: Gas chromatograph with a flame ionization detector

Method reference

Sugar Profile: AOAC 977.20, High-Pressure Liquid Chromatographic Determination of Carbohydrates in Food Products: Evaluation of Method, J.W. DeVries, J.C. Heroff, and D.C.Egberg, J. Assoc. Off. Anal. Chem. 62 p1292 (1979)

Sugar Alcohol Screen: Internally-developed method.

Reportable unit


Sample size requirements

40 grams

Information required by submitter

Please supply an estimate of the sugar and salt levels, if available.

Additional information

Sugar alcohols co-elute/interfere with simple sugars during the analysis of the Sugar Profile, resulting in the need to run both Sugar Profile by HPLC Test and Sugar Alcohol Screen (Gas Chromatography) in order to determine total sugar in samples that contain added or naturally occurring sugar alcohols.

The simple sugar(s) experiencing interference during the Sugar Profile Test will be reported under the Sugar Alcohol Screen.

Total Sugars will be reported as a single line item on the Final Report, listed as “Sugars, Total by Calculation” which is a sum of all reportable simple sugars (fructose, galactose, glucose, sucrose, maltose, lactose) across both the Sugar Profile Test and the Sugar Alcohol Screen.

*Prices and offerings are subject to change without notice.

Common Sugars, Total by Calculation Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.