Test Price:
$149 per analysis
Acceptable Matrices:
This method is applicable to grains, ingredients, and lyophilized-finished products.
Detection Limit:
The range of this method is 2 to 64% resistant starch.
Sample Size Requirements:
5 grams
Equipment:
Spectrophotometer
Reportable Units:
%
Turnaround Time:
7-9 business days
Is rush testing available?
Yes
Method Reference:
AOAC 2002.02
Method Description:
Samples are incubated with pancreatic a-amylase and amyloglucosidase (AMG) and further treated to remove the nonresistant starch. The resistant starch is then dissolved in KOH, neutralized with acetate buffer, and is quantitatively hydrolyzed to glucose with AMG. Glucose is measured with glucose oxidase/peroxidase reagent (GOP) on a spectrophotometer. The absorbance of this solution at 510 nm is proportional to the resistant starch content of the sample.
Information Required by Submitter:
Please provide estimates for samples containing high (> 10%) resistant starch. The method must be adjusted slightly for samples with high resistant starch.
Additional Information:
This method measures RS2 (granular) and RS3 (retrograded) starches. RS1 starches are not detected by this method. Applicability of this method to RS4 (chemically-cross linked) type starches must be determined on a case-by-case basis.
Contact Us:
If you have questions about our testing services, please contact us at +1 (800) 245-5615.