Resistant Starch Test

Price of the test:

$149 per analysis

Acceptable Matrices:

This method is applicable to grains, ingredients and lyophilized finished products.

Limit of Detection:

The range of this method is 2 to 64% Resistant Starch.

Sample Size Requirements:

5 grams

Equipment:

Spectrophotometer

Reportable Units:

%

Turn Around Time:

7-9 Business Days

Is Rush Available?

Yes

Method Reference:

AOAC 2002.02

Method Description:

Samples are incubated with pancreatic a-amylase and amyloglucosidase (AMG) and further treated to remove the non-resistant starch. The resistant starch is then dissolved in KOH, neutralized with acetate buffer, and then is quantitatively hydrolyzed to glucose with AMG. Glucose is measured with glucose oxidase/peroxidase reagent (GOP) on a spectrophotometer. The absorbance of this solution at 510 nm is proportional to the RS content of the sample.

Information Required by Submitter:

Please provide estimates for samples containing high (> 10%) resistant starch. The method must be adjusted slightly for samples with high resistant starch.

Additional Information:

This method measures RS2 (granular) and RS3 (retrograded) starches. RS1 starches are not detected by this method. Applicability of this method to RS4 (chemically-cross linked) type starches must be determined on a case-by-case basis.