Price of the test:
$149 per analysis
Acceptable Matrices:
This method is applicable to grains, ingredients and lyophilized finished products.
Limit of Detection:
The range of this method is 2 to 64% Resistant Starch.
Sample Size Requirements:
5 grams
Equipment:
Spectrophotometer
Reportable Units:
%
Turn Around Time:
7-9 Business Days
Is Rush Available?
Yes
Method Reference:
AOAC 2002.02
Method Description:
Samples are incubated with pancreatic a-amylase and amyloglucosidase (AMG) and further treated to remove the non-resistant starch. The resistant starch is then dissolved in KOH, neutralized with acetate buffer, and then is quantitatively hydrolyzed to glucose with AMG. Glucose is measured with glucose oxidase/peroxidase reagent (GOP) on a spectrophotometer. The absorbance of this solution at 510 nm is proportional to the RS content of the sample.
Information Required by Submitter:
Please provide estimates for samples containing high (> 10%) resistant starch. The method must be adjusted slightly for samples with high resistant starch.
Additional Information:
This method measures RS2 (granular) and RS3 (retrograded) starches. RS1 starches are not detected by this method. Applicability of this method to RS4 (chemically-cross linked) type starches must be determined on a case-by-case basis.