Technical data sheet
Samples are incubated with pancreatic a-amylase and amyloglucosidase (AMG) and further treated to remove the nonresistant starch. The resistant starch is then dissolved in KOH, neutralized with acetate buffer, and is quantitatively hydrolyzed to glucose with AMG. Glucose is measured with glucose oxidase/peroxidase reagent (GOP) on a spectrophotometer. The absorbance of this solution at 510 nm is proportional to the resistant starch content of the sample.
Plant and starch materials.
Matrices that have the potential to gelatinize in the presence of water (i.e. high concentrations of psyllium or konjac).
Limit of quantitation
2% to 64%
Sample size requirements
Information required by submitter
Please supply estimates
This method measures RS2 (granular) and RS3 (retrograded) starches. RS1 starches are not detected by this method. Applicability of this method to RS4 (chemically-cross linked) type starches must be determined on a case-by-case basis.