Price of the test:
$149 per analysis
This method is applicable to grains, ingredients and lyophilized finished products.
Limit of Detection:
The range of this method is 2 to 64% Resistant Starch.
Sample Size Requirements:
Turn Around Time:
7-9 Business Days
Is Rush Available?
Samples are incubated with pancreatic a-amylase and amyloglucosidase (AMG) and further treated to remove the non-resistant starch. The resistant starch is then dissolved in KOH, neutralized with acetate buffer, and then is quantitatively hydrolyzed to glucose with AMG. Glucose is measured with glucose oxidase/peroxidase reagent (GOP) on a spectrophotometer. The absorbance of this solution at 510 nm is proportional to the RS content of the sample.
Information Required by Submitter:
Please provide estimates for samples containing high (> 10%) resistant starch. The method must be adjusted slightly for samples with high resistant starch.
This method measures RS2 (granular) and RS3 (retrograded) starches. RS1 starches are not detected by this method. Applicability of this method to RS4 (chemically-cross linked) type starches must be determined on a case-by-case basis.