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Protease Activity Test

  • Price

    $285 per analysis

  • Turnaround time

    12 - 15 Business Days

  • Rush


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Protease Activity Test

Technical data sheet


Method description

Extracted samples are reacted with protease specific substrate. Colored product is released, and protease activity is measured on a spectrophotometer.

Acceptable matrices

Cereal grains and enzyme preps

Unacceptable matrices

Highly colored samples

Limit of quantitation

0.2 U/g


Waterbath, spectrophotometer, centrifuge

Method reference

Megazyme S-AZCAS

Reportable unit


Sample size requirements

10 g

Information required by submitter

Please supply estimates

Additional information

Estimates on enzyme preps appreciated. Some conversion factors exist, please contact us with questions.

*Prices and offerings are subject to change without notice.

Common Protease Activity Test Testing Questions

To measure the levels of protease in food products/ingredients.

Extracted samples are reacted with protease-specific substrate. Colored product is released, and protease activity is measured on a spectrophotometer.

Proteolytic enzymes are generally considered safe but can cause side effects in some people.

These enzymes are used to reduce the mixing time, decrease dough consistency and uniformity, regulate the gluten strength in bread, and improve the texture and flavor.

Higher levels of protease can cause side effects such as nausea, diarrhea, and abdominal cramping.

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.