$255 per analysis
Cereal grains, doughs, pastas, and vegetables
Sample Size Requirements:
UV/Vis spectrophotometer, homogenizer, microplate reader
1 Unit = 1 nM Catechol converted to 1,2 benzoquinone per minute under assay conditions.
7-9 business days
Is rush testing available?
Anderson JV and Morris CF (2001) Crop Science 41:1697-1705.; AACC 22-85
Samples are homogenized and enzymes are extracted into a cold, pH 6.5 buffer. A protein determination by the Bradford method is performed on the enzyme extract (supernatant) to normalize subsequent enzyme results to the efficiency of the protein extraction. The enzyme extract is mixed with catechol and Polyphenol Oxidase activity is measured spectrophotometrically by the rate of formation of 1,2 benzoquinone at 410nm.
Information Required by Submitter:
Estimates of activity (if available) and composition of the sample matrix.
Please keep and ship samples frozen, if possible.
If you have questions about our testing services, please contact us at +1 (800) 245-5615.