Technical data sheet
Samples are homogenized and enzymes are extracted into a cold, pH 6.5 buffer. A protein determination by the Bradford method is performed on the enzyme extract (supernatant) to normalize subsequent enzyme results to the efficiency of the protein extraction. The enzyme extract is mixed with catechol and Polyphenol Oxidase activity is measured spectrophotometrically by the rate of formation of 1,2 benzoquinone at 410nm.
Cereal grains, doughs, pastas, and vegetables
UV/Vis spectrophotometer, homogenizer, microplate reader
Anderson JV and Morris CF (2001) Crop Science 41:1697-1705.; AACC 22-85
1 Unit = 1 nM Catechol converted to 1,2 benzoquinone per minute under assay conditions.
Sample size requirements
Information required by submitter
Estimates of activity (if available) and composition of the sample matrix.
Please keep and ship samples frozen, if possible.