$270 per analysis
Acceptable Matrices:
Foods and Food Ingredients
Unacceptable Matrices:
Limit of Detection:
0.010 mg/kg
Sample Size Requirements:
20 grams
Equipment:
QTRAP LC-MS/MS
Reportable Units:
ppm (mg/kg)
Turn Around Time:
7-9 Business Days (matrix dependent)
5 Business Days for Rush (matrix dependent)
Is Rush Available?
Yes
Method Reference:
Please contact for Method Details
Method Description:
Glyphosate is a broad spectrum herbicide applied widely to many crops such as corn, soybean, and oats. It may also be applied as a drying agent prior to harvesting crops. Glyphosate may degrade into aminomethylphosphonic acid (AMPA) via microbial degradation in the soil. AMPA may be taken up in biota, but often is measured at much lower concentrations than glyphosate. Glyphosate and AMPA are highly polar and zwitterionic compounds requiring unique extraction and analytical chromatographic conditions apart from a traditional multi-residue analysis (MRA) method. The extraction requires the use of an acidified solvent containing a chelating agent to first precipitate the protein, and then to bind any metal ions which may interact with the target analytes. A solid phase cleanup step is required to remove phospholipids (i.e. lecithin) and other non-polar coextractives. These may build up on the analytical column and affect chromatographic performance, if not removed prior to LCMS acquisition. A mixed-mode analytical column is required to successfully chromatograph glyphosate and AMPA. Because of the column characteristics, and the four pKas present on each of the target analytes, the pH of the mobile phase must be adjusted accordingly.