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Peroxidase Activity Test

  • 271 per analysis
  • |
  • 7-9 business days
  • |
  • Rush Available
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Peroxidase Activity Test

Technical data sheet


Method description

Samples are homogenized and enzymes are extracted into a cold, pH 6.5 buffer.  A protein determination by the Bradford method is performed on the enzyme extract (supernatant) to normalize subsequent enzyme results to the efficiency of the protein extraction.  The enzyme extract is mixed with hydrogen peroxide, guaiacol, and calcium. Peroxidase activity is measured spectrophotometrically by the rate of formation of tetraguaiacol at 470nm.

Acceptable matrices

Cereal grains, doughs, pasta, and vegetables


UV/Vis spectrophotometer, homogenizer, microplate reader

Method reference

Britton Chance, A.C. Maehly, Method in Enzymology, Assay of catalases and peroxidases. Vol 2, Academic Press, New York, 1955, pp. 764-775.

Reportable unit

1 Unit = 0.5 μM of Guaiacol consumed per minute under assay conditions.

Sample size requirements

20 grams

Information required by submitter

Estimates of activity (if available) and composition of the sample matrix.

Additional information

Peroxidase is an enzyme that can catalyze the cross-linking of phenolic residues by H2O2 resulting in undesirable color changes (graying) and the potential generation of off-flavors in a food product. Determining the average level of peroxidase in different cultivars can help guide breeding decisions. Additionally, because peroxidases are relatively stable enzymes, they can serve as an indicator for the efficacy of enzyme inactivating treatments in grains and flours (heat treatment, chemical treatment, etc.)

Please keep and ship samples frozen, if possible.

*Prices and offerings are subject to change without notice.

Common Peroxidase Activity Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.