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Nitrogen Test

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Technical data sheet

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Method description

Total nitrogen is determined using a combustion instrument operating on the Dumas principle. This method is universal for the determination of nitrogen content and can be used for nearly all food products. Pure oils and shortenings may cause fires in the instrument and should be assayed by the Kjeldahl method.

The sample is completely combusted at high temperature in the presence of pure O2 (99.9%). Combustion products are H2O, CO2, NOx, and N2. Water vapor, oxygen, and CO2 are removed from the resulting gas stream by a series of thermoelectric coolers and chemical sorbents. NOx is reduced to N2 when passed through a hot column packed with copper. The resulting N2 is passed to a thermal conductivity detector, where it is quantified using helium as the carrier gas.

Acceptable matrices

This method is universal for the determination of nitrogen content and can be used for nearly all food products.

Unacceptable matrices

Pure oils and shortenings cause fires in the instrument and should be assayed by the Kjeldahl method.

Equipment

Protein/Nitrogen by combustion analyzer.

Method reference

AACC 46-30; AOAC 992.15

Reportable unit

% Nitrogen

Sample size requirements

Please provide a sample of at least 20g to ensure results representative of the sample submitted.

*Prices and offerings are subject to change without notice.

Common Nitrogen Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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