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Moisture (Karl Fischer Titration) Test

  • Price

    $60 per analysis

  • Turnaround time

    7 - 9 Business Days

  • Rush


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Moisture (Karl Fischer Titration) Test

Technical data sheet


Method description

Moisture by Karl Fischer is determined by automated potentiometric titration with an iodine and sulfur dioxide reagent. Moisture is extracted into an appropriate solvent and is titrated volumetrically (as opposed to colorimetric titration). This method is applicable to high-fat samples or samples that contain compounds that may volatilize when heated or subjected to vacuum (e.g. alcohol, glycerine, etc.)

Acceptable matrices

This method is applicable to fats and oils, peanut butter, pet foods, solvents, and salad dressings with high fat content (greater than 20%).

Unacceptable matrices

Organic acids, ketones, or sugars such as sorbitol.

Limit of quantitation



Standard titration set up.

Method reference

AOAC 984.20, 967.19, 977.10 and AOCS Ca 2e-55.

Reportable unit


Sample size requirements

15 g

Information required by submitter

Please supply estimates

Additional information

Moisture in most food materials can be determined by loss on drying using a Gravimetric Moisture method. For samples high in fat or that contain components that will volatilize under the conditions of the Gravimetric Moisture assays, the Karl Fischer titration may be more appropriate. Note that chemically bound water molecules may be incompletely detected by this method.

*Prices and offerings are subject to change without notice.

Common Moisture (Karl Fischer Titration) Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.