Listeria genus (PCR) Test

Method description

GENE-UP® Listeria spp. 2 (LIS 2) is a real-time Polymerase Chain Reaction (PCR) assay for the detection of Salmonella in food, pet food and environmental samples.

Acceptable matrices

Food and Environmental Samples
(For environmental samples, only 25g swaps are accepted)

Unacceptable matrices

Cosmetics, non-food samples

Equipment

Biomerieux GENE-UP

Method reference

AOAC 2019.10

Reportable unit

For 25g test
Suspect positive or negative/sample size (swab or 25g)

For 125g (Composite) test
Suspect positive or negative/125g

Sample size requirements

For 25g test
1 swab/test
50g – 100g
*100g required if matrix verification is needed

For 125g (Composite) test
Minimum of 500g

Additional information

GENE-UP® Listeria spp. 2 (LIS 2) The assay is a molecular assay that uses Fluorescence Resonance Energy Transfer (FRET) technology during PCR and the amplified target is detected by the GENE‑UP® Thermocycler.

PCR and ELFA Method Comparison – Which method should I choose?

Both test methods will provide accurate results. See the table below for a high-level test comparison.