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Lipoxygenase Activity Test

  • 271 per analysis
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  • 7-9 Business Days
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  • Turnaround Time Delay
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  • Rush Available
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Technical data sheet

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Method description

Samples are homogenized and enzymes are extracted into a cold, pH 6.5 buffer.  A protein determination by the Method of Bradford is performed on the enzyme extract (supernatant) to normalize subsequent enzyme results to the efficiency of the protein extraction.  The enzyme extract is mixed with linoleic acid or arachidonic acid.  Lipoxygenase activity is measured spectrophotometrically by the rate of formation of the fatty acid hydroperoxide at 234nm.

Acceptable matrices

cereal grains, doughs, pastas, and vegetables

Equipment

UV/Vis spectrophotometer, homogenizer, microplate reader

Method reference

Axelrod B, Cheesbrough TM, and Laakso S (1981) Lipoxygenase from soybeans, In “Methods in Enzymology”, Vol. 71. (Ed.) JM Lowenstein.  Academic Press, New York

Ben-Aziz A, Grossman S, Ascarelli I, and Budowski P (1970) Linoleate oxidation induced by lipoxygenase and heme proteins: a direct spectrophotometric assay.  Anal. Biochem. 34:8

Reportable unit

units per gram.
One unit is defined as the change in absorbance at 234nm per minute from the hydroperoxidation of the cis, cis-1,4 pentadiene containing fatty acid.
One unit is equivalent to the oxidation of 0.12 micromoles of linoleic acid.

Sample size requirements

20 grams

Information required by submitter

Estimates of activity if available.
Composition of sample matrix.

Additional information

Please keep and ship samples frozen if possible.

*Prices and offerings are subject to change without notice.

Common Lipoxygenase Activity Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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