Price of the test:
$255 per analysis
Acceptable Matrices:
cereal grains, doughs, pastas, and vegetables
Limit of Detection:
0.01 units/gram
Sample Size Requirements:
20 grams
Equipment:
UV/Vis spectrophotometer, homogenizer, microplate reader
Reportable Units:
units per gram.
One unit is defined as the change in absorbance at 234nm per minute from the hydroperoxidation of the cis, cis-1,4 pentadiene containing fatty acid.
One unit is equivalent to the oxidation of 0.12 micromoles of linoleic acid.
Turn Around Time:
7-9 Business Days
Is Rush Available?
Yes
Method Reference:
Axelrod B, Cheesbrough TM, and Laakso S (1981) Lipoxygenase from soybeans, In “Methods in Enzymology”, Vol. 71. (Ed.) JM Lowenstein. Academic Press, New York
Ben-Aziz A, Grossman S, Ascarelli I, and Budowski P (1970) Linoleate oxidation induced by lipoxygenase and heme proteins: a direct spectrophotometric assay. Anal. Biochem. 34:8
Method Description:
Samples are homogenized and enzymes are extracted into a cold, pH 6.5 buffer. A protein determination by the Method of Bradford is performed on the enzyme extract (supernatant) to normalize subsequent enzyme results to the efficiency of the protein extraction. The enzyme extract is mixed with linoleic acid or arachidonic acid. Lipoxygenase activity is measured spectrophotometrically by the rate of formation of the fatty acid hydroperoxide at 234nm.
Information Required by Submitter:
Estimates of activity if available.
Composition of sample matrix.
Additional Information:
Please keep and ship samples frozen if possible.