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Lipase Activity Test

  • Price

    $315 per analysis

  • Turnaround time

    12 - 15 Business Days

  • Rush


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Lipase Activity Test

Technical data sheet


Method description

Samples are homogenized in a cold phosphate buffered saline solution (PBS), pH 8.5 and then centrifuged. A protein determination by the Method of Bradford is performed on the enzyme extract (supernatant) to normalize subsequent enzyme results to the efficiency of the protein extraction. The enzyme extract is incubated at 37C with a saturating amount of triglyceride that contains sulfhydryl groups substituted for the normal carboxylic acid groups on the fatty acids. As the extracted lipase in the supernatant cleaves the modified fatty acids from the glycerol backbone, the sulfhydryl groups are made available for reaction with a color reagent (DTNB). The color reagant absorbs visible light at 412nm in direct proportion to the number of sulfhydryl groups exposed by the lipase. The appearance of absorbance with time of reaction is monitored kinetically. The initial velocity of the reaction is determined from the reaction rate.

Acceptable matrices

cereal grains, doughs, pastas, and vegetables

Limit of quantitation

2783 Units/g


Waterbath, spectrophotometer, centrifuge

Method reference

BioAssay Systems Lipase Assay Kit (DLPS-100)

Reportable unit

Units per gram. One unit of enzyme catalyzes the cleavage of 1 µmole of substrate per minute under the assay conditions (pH 8.5).

Sample size requirements

20 g

Information required by submitter

Please supply estimates

*Prices and offerings are subject to change without notice.

Common Lipase Activity Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.