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Gluten/Gliadin Allergens Test

  • Price

    $160 per analysis

  • Turnaround time

    7-9 Business Days

  • Rush


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Gluten/Gliadin Allergens Test

Technical data sheet


Method description

Samples are extracted based on matrix. Standards and samples are added to the wells, and present gliadin will bind to specific capture antibodies. The result is an antibody-antigen-complex. Components not bound by the antibodies are then removed in a washing step. Antibody conjugated to peroxidase is added and this antibody-conjugate is bound to the Ab-Ag complex. Any unbound enzyme conjugate is then removed in a washing step. Enzyme substrate and chromogen are added to the wells and incubated. Bound enzyme conjugate converts the colorless chromagen into a blue product. The addition of the stop reagent leads to a color change from blue to yellow. The measurement is made photometrically at 450 nm. The absorbance is proportional to the gliadin concentration of the sample. This result is then multiplied by 2 in order to obtain the gluten concentration (gliadin usually represents 50% of the proteins present in gluten).

Acceptable matrices

Raw and processed foods

Unacceptable matrices

Matrices that are incapable of dissolving in an 80% alcohol solution.

Concentrated food additives, colors, and flavors may cause interference in this analysis.

Hydrolyzed and fermented proteins may not be detected using ELISA methods for allergen testing. Due to the breakdown of the proteins into small peptides or amino acids, they may become undetectable by this assay, but still could be allergenic and cause an allergic reaction.

Limit of quantitation

Lower LOQ = 10 ppm

Upper LOQ = 80 ppm


Microwell Strip Reader

Method reference


Reportable unit


Sample size requirements

100 grams

Additional information

This method is intended for the quantitative analysis of prolamins (plant storage proteins having high content of the amino acid proline) originating from wheat (gliadin), rye (secalin), and barley (hordein).

*Prices and offerings are subject to change without notice.

Common Gluten/Gliadin Allergens Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

Our LOQ is 10 ppb (parts per billion).

With all regulation questions, we recommend that each of our clients works directly with a lawyer or consultant that is knowledgable for proper labeling. This test is a great part of a toolkit to ensure that products are properly labeled and safe for gluten sensitive individuals, however, Gluten Free labeling is more involved than a single test.

Our LOQ is 10 ppb (parts per billion).

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.