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Free Fatty Acids Test

  • Price

    $105 per analysis

  • Turnaround time

    7 - 9 Business Days

  • Rush


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Free Fatty Acids Test

Technical data sheet


Method description

The fat in a sample is extracted into an organic solvent and titrated with sodium hydroxide. Rancidity is calculated as percent oleic acid for most fats and oils. Coconut and palm kernel oils are calculated as percent lauric acid, and palm oil is calculated as percent palmitic acid.

Acceptable matrices

Fats and oils. Food products containing at least 3% fat.

Unacceptable matrices

Dairy, egg yolks, dough, rice bran oil, lecithin, fish oil, soup, products containing less than 3% fat

Limit of quantitation

Fats and Oils - 0.01%, Foods - 0.15%. The limit of quantitation will vary based on the sample weight analyzed.


Electronic titrator

Method reference

AOCS CA 5a-40

Reportable unit

Samples reported as % FFA as oleic acid. May be reported as % lauric acid and % palmitic acid upon request.

Sample size requirements

100 g

Information required by submitter

Please supply estimates

Additional information

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. This test provides a measure of hydrolytic rancidity in an oil or food product. Hydrolytic rancidity involves chemical enzymatic hydrolysis of fats into free fatty acids and glycerol.

*Prices and offerings are subject to change without notice.

Common Free Fatty Acids Test Testing Questions

Determination of FFA content is an important analysis for evaluating the quality of raw material and its degradation during storage and throughout shelf life.

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

Free fatty acids form during the frying process due to the presence of moisture in foods exposed to oil. If the frying rate is insufficient to drive water from food at a sufficient rate, the FFS content increases rapidly.

There is no “normal” value for FFA, it varies from sample to sample and what conditions it has been in.

There is no relationship between FFA results and PV results.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

While regulations can vary, generally, Vitamin D and our Metal Screen I Package (Calcium, Iron, Potassium, and Sodium) are most commonly requested when creating a nutrition label.

This test provides a measure of hydrolytic rancidity in an oil or food product. Hydrolytic rancidity involves chemical enzymatic hydrolysis of fats into free fatty acids and glycerol.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.