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Home / Vitamin B9 (Folic Acid – LC/MS) – Test

Vitamin B9 (Folic Acid - LC/MS) - Test

  • Price

    $190 per analysis

  • Turnaround time

    7 - 9 Business Days

  • Rush


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Vitamin B9 (Folic Acid - LC/MS) - Test

Technical data sheet


Method description

A homogenized sample is weighed and internal standard and phosphate buffer are added. Amylase and protease enzymes are added to further break down the matrix for extraction of the folates. Conjugase is used to de-conjugate the polyglutamate forms of folates to mono-glutamates. The sample is centrifuged, filtered and cleaned using SPE. The folates are analyzed by UPLC-MS/MS. Folic Acid is quantified. Isotopically labeled internal standards are used to control matrix to matrix variation.

Acceptable matrices

All cereal products, flours, milk and soy-based products, infant formulas and baby food composite, vegetables, fruits, protein powder and shake, vitamin concentrates (up to 2 % folates), peanuts, peanut butter and other food matrices.

Unacceptable matrices

Gum, encapsulated vitamins, premixes and supplements above 2%

Limit of quantitation

10µg/100g - 2,000,000 µg/100g (2 % total folates)


High-Performance Liquid Chromatography with Fluorescence Detection

Method reference

AOAC 2011.06

Reportable unit


Sample size requirements

5 g

Information required by submitter

Please supply estimates

Additional information

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. This method is not designed for use on vitamin concentrates, and the vitamer 5-methyl tetrahydrofolate will not be quantified.

*Prices and offerings are subject to change without notice.

Common Vitamin B9 (Folic Acid - LC/MS) - Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.