Technical data sheet
A homogenized sample is weighed and internal standard and phosphate buffer are added. Amylase and protease enzymes are added to further break down the matrix for extraction of the folates. Conjugase is used to de-conjugate the polyglutamate forms of folates to mono-glutamates. The sample is centrifuged, filtered and cleaned using SPE. The folates are analyzed by UPLC-MS/MS. Folic acid is quantified. Isotopically labeled internal standards are used to control matrix to matrix variation.
This method is applicable to all cereal products, flours and dough products, milk and soy-based products, infant formulas and baby food composites, vegetables, fruits, protein powders and shakes, peanuts and peanut-based products.
Ultra-high Performance Liquid Chromatography – MS/MS detection
Sample size requirements
10 grams minimum