Vitamin B9 (Folic Acid - LC/MS) - Test
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160 per analysis | -
7-9 Business Days | -
Rush Available
Technical data sheet
PrintMethod description
A homogenized sample is weighed and internal standard and phosphate buffer are added. Amylase and protease enzymes are added to further break down the matrix for extraction of the folates. Conjugase is used to de-conjugate the polyglutamate forms of folates to mono-glutamates. The sample is centrifuged, filtered and cleaned using SPE. The folates are analyzed by UPLC-MS/MS. Folic acid is quantified. Isotopically labeled internal standards are used to control matrix to matrix variation.
Acceptable matrices
This method is applicable to all cereal products, flours and dough products, milk and soy-based products, infant formulas and baby food composites, vegetables, fruits, protein powders and shakes, peanuts and peanut-based products.
Equipment
Ultra-high Performance Liquid Chromatography – MS/MS detection
Method reference
AOAC 2011.06
Reportable unit
µg/100g
Sample size requirements
10 grams minimum
Common Vitamin B9 (Folic Acid - LC/MS) - Test Testing Questions
While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.
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Medallion Labs
A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.
