Price of the test:
$140 per analysis
Acceptable Matrices:
This method is applicable to all cereal products, flours and dough products, milk and soy-based products, infant formulas and baby food composites, vegetables, fruits, protein powders and shakes, peanuts and peanut-based products.
Limit of Detection:
10µg/100g – 2,000,000 µg/100g (2 % total folates)
Sample Size Requirements:
10 grams minimum
Equipment:
Ultra-high Performance Liquid Chromatography – MS/MS detection
Reportable Units:
µg/100g
Turn Around Time:
7-9 Business Days
Is Rush Available?
Yes
Method Reference:
AOAC 2011.06
Method Description:
A homogenized sample is weighed and internal standard and phosphate buffer are added. Amylase and protease enzymes are added to further break down the matrix for extraction of the folates. Conjugase is used to de-conjugate the polyglutamate forms of folates to mono-glutamates. The sample is centrifuged, filtered and cleaned using SPE. The folates are analyzed by UPLC-MS/MS. Two folate vitamers, folic acid and 5-methyl tetrahydrofolate, are quantified. Isotopically labeled internal standards are used to control matrix to matrix variation.