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Home / Vitamin B9 (Total Folates – Microbial Method) Test

Vitamin B9 (Total Folates - Microbial Method) Test

  • Price

    $170 per analysis

  • Turnaround time

    7 - 9 Business Days

  • Rush


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Vitamin B9 (Total Folates - Microbial Method) Test

Technical data sheet


Method description

A homogenous sample is dispersed in a phosphate buffer solution at pH 7. Total folates are extracted from the sample using a triple enzyme system. A protease and an amylase are used to digest the food matrix and aid in the release of folates. Rat plasma conjugase is used to hydrolyze the polyglutamate side chain. The assay microorganism, Lactobacillus casei, subsp. rhamnosus (ATCC no. 7469), utilizes total folate derivatives for growth. The sample extract is diluted with an assay medium containing all required growth nutrients for the microorganism except for folates, inoculated with the microorganism, and incubated for 24 hours at 37°C. The growth response of the microorganism (measured as turbidity at 610 nm) in the sample is compared quantitatively to that of known standard solutions of folic acid.

Acceptable matrices

Cereals, fruits, vegetables, flours, grains, bread, pasta, bars, vitamin drink supplements and concentrates that contain added or naturally occurring folates.

Unacceptable matrices

Dairy, meats, eggs, fats and oils, plant based protein, soup, seafood. Premixes/concentrates/supplements above 2%.

Limit of quantitation

0.8 µg/100g


Automated pipettor system Automated plate reader system

Method reference

AOAC 944.12 and AACC 86-47.01

Reportable unit


Sample size requirements

10 g

Information required by submitter

Please supply estimates

Additional information

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. This method is not designed for use on vitamin concentrates.

*Prices and offerings are subject to change without notice.

Common Vitamin B9 (Total Folates - Microbial Method) Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.