Technical data sheet
A homogenous sample is dispersed in a phosphate buffer solution at pH 7. Total folates are extracted from the sample using a triple enzyme system. A protease and an amylase are used to digest the food matrix and aid in the release of folates. Rat plasma conjugase is used to hydrolyze the polyglutamate side chain. The assay microorganism, Lactobacillus casei, subsp. rhamnosus (ATCC no. 7469), utilizes total folate derivatives for growth. The sample extract is diluted with an assay medium containing all required growth nutrients for the microorganism except for folates, inoculated with the microorganism, and incubated for 24 hours at 37°C. The growth response of the microorganism (measured as turbidity at 610 nm) in the sample is compared quantitatively to that of known standard solutions of folic acid.
Cereals, fruits, vegetables, flours, grains, bread, pasta, bars, vitamin drink supplements and concentrates that contain added or naturally occurring folates.
Dairy, meats, eggs, fats and oils, plant based protein, soup, seafood. Premixes/concentrates/supplements above 2%.
Limit of quantitation
Automated pipettor system Automated plate reader system
AOAC 944.12 and AACC 86-47.01
Sample size requirements
Information required by submitter
Please supply estimates
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. This method is not designed for use on vitamin concentrates.