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Fiber (Total - Resistant Oligosaccharides Method) Test

  • Price

    $500 per analysis

  • Turnaround time

    7 - 9 Business Days

  • Rush

    Available

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Fiber (Total - Resistant Oligosaccharides Method) Test

Technical data sheet

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Method description

This method determines traditional total dietary fiber (TDF) and digestion-resistant oligosaccharides (ROs) in both food ingredients and food products. Samples (defatted if necessary) are first run by a traditional dietary fiber method, in which digestion with three enzymes (protease, amylase, and amyloglucosidase) is carried out in an MES-Tris buffer and traditional dietary fiber is precipitated with ethanol. The final filtrate solution from the traditional total dietary fiber analysis is collected after filtering off the precipitated dietary fiber. Resistant oligosaccharides are soluble in this alcoholic solution, so in order to quantitate them, the filtrate is evaporated to 20 mL. Deionized through a series of anion and cation exchange columns to remove interferences from buffers, salts, amino acids, peptides, and protein and then reconcentrated to 10 mL. The solution is mixed well and syringe filtered prior to analysis by size exclusion HPLC. The size exclusion HPLC columns separate sample solution components into peaks based on molecular weight. The peaks corresponding to oligosaccharides larger than DP2 are quantitated, summed, and reported as total resistant oligosaccharides.

Acceptable matrices

Food and Food Ingredients

Unacceptable matrices

Baking powder, Samples containing more than 2% stevia

Limit of quantitation

Total Dietary Fiber - 0.1% Resistant Oligosaccharides (RO) - 0.1%

Equipment

General Gravimetric Analytical Chemistry Equipment HPLC-Refractive Index Detector

Method reference

AOAC 2001.03 with AOAC 991.43 digestion

Reportable unit

%

Sample size requirements

25 g

Information required by submitter

please supply expected estimates. Indicate matrix type, and indicate if samples are high in fat (>5% fat). Indicate whether the sample contains stevia, fructans (inulin or fructooligosaccharides (FOS)), psyllium, flax, and gum (i.e. guar gum)

Additional information

This test is ISO/IEC 17025 accredited through A2LA, Certificate #2769.01. Dietary Fiber is the portion of the carbohydrates which is not digested in the human gut. It reports the sum of both soluble and insoluble components. These methods are applicable to all food products, however samples containing psyllium or fructans should be submitted under specific test codes. High-fat (>5%) samples require special handling. Resistant oligosaccharides are polysaccharides from DP3 and higher which are soluble in ethanol and are resistant to digestion by amylase under the conditions of the traditional dietary fiber analysis (AOAC 991.43). In the past, this analysis has gone by names such as “fibersol” and “resistant maltodextrins.” At Medallion Labs, we feel that these terms are limiting because we know that other polysaccharides, such as fructans are also included in this result. We are using AOAC method 2001.03, but have renamed the result “resistant oligosaccharides” because we feel this is the proper, general classification for the molecules that are being measured.

*Prices and offerings are subject to change without notice.

Common Fiber (Total - Resistant Oligosaccharides Method) Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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