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Fiber (Total - CODEX Definition) Test

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Fiber (Total - CODEX Definition) Test

Technical data sheet


Method description

Samples (defatted if necessary) are digested 16 hours in a manner that simulates the human digestive system with 3 enzymes (pancreatic alpha-amylase, protease, and amyloglucosidase) to remove starch and protein. Ethanol is added to precipitate high molecular weight soluble dietary fiber. The resulting solution is filtered and the residue is dried and weighed. Protein and ash are determined on the residue. The filtrate is captured, concentrated, and analyzed via HPLC to determine the value of low molecular weight soluble dietary fiber (LMWSF).

Total dietary fiber (TDF) is calculated as the weight of the residue minus protein and ash plus the low molecular weight soluble fiber determined by HPLC analysis.

Acceptable matrices

All food products

Unacceptable matrices

Stevia can cause interference with the chromatography. Samples containing >2% stevia will damage the instrument therefore cannot be run through this method. Glucomannan

Limit of quantitation




Method reference

AOAC 2009.01

Reportable unit


Sample size requirements

40 grams

Information required by submitter

Please supply expected estimates. Indicate matrix type and indicate if samples are high in fat (>5%). Indicate whether the sample contains fructans (inulin or fructooligosaccarides (FOS)), or psyllium, flax and gum (i.e. guar gum).

Additional information

Measures total dietary fiber including resistant starch and low molecular weight non-digestible oligosaccharides of DP =3.  If a breakout of soluble and insoluble fiber as well as total dietary fiber is desired, please choose our Total, Soluble, and Insoluble Dietary Fiber CODEX Definition method.

Please include notification for samples containing DP2 and DP3 carbohydrates (Isomalto-oligosaccharides (IMO), Maltiltol, Lactose).

Dietary Fiber is the portion of the carbohydrates which is not digested in the human gut. It is composed of both soluble and insoluble components. High-fat (>5%) samples require special handling.

*Prices and offerings are subject to change without notice.

Common Fiber (Total - CODEX Definition) Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.