Fiber (Gravimetric) - Total, Soluble, Insoluble Test
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Price
$240 per analysis
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Turnaround time 7 – 9 Business Days
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Rush Available
Technical data sheet
Print- Method description
Samples (defatted if necessary) are digested with three enzymes (alpha-amylase, protease and amyloglucosidase) to remove starch and protein. Any solid particles not in solution after digestion are considered insoluble dietary fiber and are filtered off and measured gravimetrically. The filtrate from this first filtration is collected and ethanol is added to precipitate the soluble dietary fiber. The soluble fraction is then filtered and measured gravimetrically. All residues are corrected for ash and protein content. Total dietary fiber is calculated as the sum of the insoluble and soluble percentages.
- Acceptable matrices
Food and Food Ingredients
- Unacceptable matrices
Baking powder
- Limit of quantitation
Total Dietary Fiber - 0.2% Insoluble Fiber – 0.1% Soluble Dietary Fiber-0.2%
- Equipment
Standard Gravimetric Analytical Chemistry Equipment
- Method reference
AOAC 991.43 with modifications
- Reportable unit
%
- Sample size requirements
25 g
- Information required by submitter
please supply expected estimates. Indicate matrix type, and indicate if samples are high in fat (>5% fat). Indicate whether the sample contains stevia, fructans (inulin or fructooligosaccharides (FOS)), psyllium, flax, and gum (i.e. guar gum)
- Additional information
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. Dietary Fiber is the portion of the carbohydrates which is not digested in the human gut. It is composed of both soluble and insoluble components. These methods are applicable to all food products, however samples containing psyllium or fructans should be submitted under specific test codes. High-fat (>5%) samples require special handling.
Is this the most suitable fiber test for evaluating my product?
Find your fiber source(s) on the chart and use the alpha reference to see how it aligns with each method. If the alpha reference is fully inside the method circle, that method is optimal for your fiber source(s). If it's outside or touching the circle's edge, the method won't fully capture the fiber. Remember to check applicable labeling regulations for allowed fiber sources.
- AOAC Method 2001.03
AOAC Method 991.43 - AOAC Method 2017.16
AOAC Method 2022.01 - AOAC Method 985.29
AOAC Method 991.43 - AOAC Method 2009.01
AOAC Method 2011.25
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Cellulose | Beta-Glucan | Psyllium | Gums | Lignin -
Galacto-oligosaccharides-Raffinose(GOS)/Stachyose -
Galactomannan | Arabinoxylan | Glucomannan -
Resistant Starch RS4 -
Resistant Starch RS2 & RS3 -
Pectin | Alginates | Arabinogalactin | Isomalto-oligosaccharide (IMO) -
Polydextrose | Fibersol®-2 | Resistant Maltodextrins | Soluble Corn Fiber (SCF) -
Fructans (Inulin, FOS)
- Cellulose | Beta-Glucan | Psyllium | Gums | Lignin
- Galacto-oligosaccharides-Raffinose(GOS)/Stachyose
- Galactomannan | Arabinoxylan | Glucomannan
- Resistant Starch RS4
- Resistant Starch RS2 & RS3
- Pectin | Alginates | Arabinogalactin | Isomalto-oligosaccharide (IMO)
- Polydextrose | Fibersol®-2 | Resistant Maltodextrins | Soluble Corn Fiber (SCF)
- Fructans (Inulin, FOS)
- Cellulose | Beta-Glucan | Psyllium | Gums | Lignin
- Galacto-oligosaccharides-Raffinose(GOS)/Stachyose
- Galactomannan | Arabinoxylan | Glucomannan
- Resistant Starch RS4
- Resistant Starch RS2 & RS3
- Pectin | Alginates | Arabinogalactin | Isomalto-oligosaccharide (IMO)
- Polydextrose | Fibersol®-2 | Resistant Maltodextrins | Soluble Corn Fiber (SCF)
- Fructans (Inulin, FOS)
- Cellulose | Beta-Glucan | Psyllium | Gums | Lignin
- Galacto-oligosaccharides-Raffinose(GOS)/Stachyose
- Galactomannan | Arabinoxylan | Glucomannan
- Resistant Starch RS4
- Resistant Starch RS2 & RS3
- Pectin | Alginates | Arabinogalactin | Isomalto-oligosaccharide (IMO)
- Polydextrose | Fibersol®-2 | Resistant Maltodextrins | Soluble Corn Fiber (SCF)
- Fructans (Inulin, FOS)
Common Fiber (Gravimetric) - Total, Soluble, Insoluble Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
The difference between soluble and insoluble fibers is their solubility in water/ethanol. This testing uses this chemistry difference to effectively separate these fiber groups. The total fiber value is the sum of the Insoluble and Soluble portions.
Gravimetric Fiber testing is able to measure a majority of the ‘classic’ dietary fibers, such as cellulose, beta-glucans, and lignans. Because the Gravimetric test does not use HPLC instrumentation, there are many types of fiber groups and newer fiber ingredients that may be missed or only partially recovered. Several examples of these missed compounds include Raffinose, Polydextrose, and a majority of Inulin.
Our food testing experts are here to help.
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