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Englyst Starch (Glycemic Response) Test

  • Price

    $575 per analysis

  • Turnaround time

    12 - 15 Business Days

  • Rush


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Englyst Starch (Glycemic Response) Test

Technical data sheet


Method description

Sample containing starch are digested using amylase and AMG under controlled conditions (simulating human digestion). Aliquots are pulled at 20 minutes and 120 minutes, and the enzyme reaction is stopped by boiling. Glucose in both fractions is measured with GOPOD (glucose oxidase – peroxidase) on a spectrophotometer and rapidly- and slowly-digestible starch is calculated.

Acceptable matrices

Cereals, grains, starches, flours, breads, and biscuits

Unacceptable matrices

Products with no starch or very low starch content. Products high in sugar.

Limit of quantitation



Incubator, Waterbath, Spectrophotometer

Method reference

Englyst, H.N et al. 1992. Classification and measurement of nutritionally important starch fractions. European Journal of Clinical Nutrition. 46(suppl. 2), S33-S50 Englyst, H.N. et al. 1996 Brit J Nutr, 75, 749-755

Reportable unit

Results G20 and G120 are unitless. G20 (Rapidly Available Glucose) G120 (Slowly Available Glucose) Total Starch (%) Moisture (%)

Sample size requirements

50 g

Information required by submitter

Please supply estimates

Additional information

Moisture and Total starch testing will be run as part of completing the Englyst test. The purpose of this method is to screen food and ingredients for the purpose of optimizing a lower Glycemic Response. A human study is helpful once a low-glycemic formula is optimized for the most extensive support of claims.

*Prices and offerings are subject to change without notice.

Common Englyst Starch (Glycemic Response) Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

The Englyst Starch test is often considered an in-vitro version of the Glycemic Index test. A Glycemic Index test would use human subjects to measure the effects of a food product on blood sugar levels. There isn't necessarily a direct conversion between the Englyst Starch test vs. Glycemic Index.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.