Sample containing starch are digested using amylase and AMG under controlled conditions (simulating human digestion). Aliquots are pulled at 20 minutes and 120 minutes, and the enzyme reaction is stopped by boiling. Glucose in both fractions is measured with GOPOD (glucose oxidase – peroxidase) on a spectrophotometer and rapidly- and slowly-digestible starch is calculated.
Cereals, grains, starches, flours, breads, and biscuits
Products with no starch or very low starch content. Products high in sugar.
Incubator, Waterbath, Spectrophotometer
Englyst, H.N et al. 1992. Classification and measurement of nutritionally important starch fractions. European Journal of Clinical Nutrition. 46(suppl. 2), S33-S50
Englyst, H.N. et al. 1996 Brit J Nutr, 75, 749-755
Results are G20 and G120 are unitless
G20 (Rapidly Available Glucose)
G120 (Slowly Available Glucose)
Total Starch (%)
Moisture and Total starch testing will be run as part of completing the Englyst test.
The purpose of this method is to screen food and ingredients for the purpose of optimizing a lower Glycemic Response. A human study is helpful once a low-glycemic formula is optimized for the most extensive support of claims.