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Cholesterol Test

  • Price

    $150 per analysis

  • Turnaround time

    7 - 9 Business Days

  • Rush


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You can expect high-quality, reliable results time after time.

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Cholesterol Test

Technical data sheet


Method description

Cholestane is used as an internal standard for cholesterol and is added prior to hydrolysis. The components are analyzed using gas chromatography with a capillary column.

Acceptable matrices

Food and Food Ingredients

Unacceptable matrices

Concentrates of Vitamin E or forms of Vitamin E such as tocopherols, tocopheryl succinate,tocotrienols, and gums.

Limit of quantitation



Gas Chromatograph with Flame Ionization Detection

Method reference

AOAC 976.26 with modifications

Reportable unit


Sample size requirements

5 g

Information required by submitter

Please supply estimates

Additional information

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. Lipids are unbound from food samples by acid or base hydrolysis and extracted into mixed ethers. The fatty acid containing compounds are then interesterfied to form fatty acid methyl esters and free sterols. The components are separated and analyzed using a capillary column on a gas chromatograph.

*Prices and offerings are subject to change without notice.

Common Cholesterol Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.