Technical data sheet
Total glucans within a sample are solubilized in ice-cold 12 M H2SO4 and hydrolyzed to near completion in 2 M H2SO4. Remaining glucan fragments are quantitatively hydrolyzed to glucose using a mixture of exo-1,3-βglucanase and β-glucosidase. An additional sample portion undergoes hydrolysis of the α-Glucans to glucose using amyloglucosidase plus a-amylase. The glucose in both sample test portions are measured using GOPOD reagent. β-Glucan is determined by difference between the total glucans and α-glucans.
Brewers yeast, yeast extract, dried mushrooms, mushrooms
Samples containing cereal grain-based Beta Glucan and/or oat-based Beta Glucan
Limit of quantitation
Sample size requirements