Price of the test:
$255 per analysis
Acceptable Matrices:
Brewers yeast, Yeast extract, dried mushrooms, mushrooms
Unacceptable Matrices:
Samples containing Cereal Grain based Beta Glucans
Samples containing Oat based Beta Glucan
Limit of Quantitation:
1%
Sample Size Requirements:
10g
Equipment:
Waterbath, Spectrophotometer
Reportable Units:
% (w/w)
Turn Around Time:
7 – 9 Business Days
Is Rush Available?
Yes
Method Reference:
Megazyme K-YBGL 09/14
Method Description:
Total glucans within a sample are solubilized in ice cold 12 M H2SO4 and then hydrolysed to near completion in 2 M H2SO4. Remaining glucan fragments are then quantitatively hydrolysed to glucose using a mixture of exo-1,3-βglucanase and β-glucosidase. An additional sample portion undergoes hydrolysis of the α-Glucans to glucose using amyloglucosidase plus a-amylase. The glucose in both sample test portions are measured using GOPOD reagent. β-Glucan is determined by difference between the total glucans and α-glucans.