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Beta-Glucan (Mushroom, Yeast) Test

  • 271 per analysis
    • |
  • 12 - 15 Business Days
    • |
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Beta-Glucan (Mushroom, Yeast) Test
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Technical data sheet

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Method description

Total glucans within a sample are solubilized in ice-cold 12 M H2SO4 and hydrolyzed to near completion in 2 M H2SO4. Remaining glucan fragments are quantitatively hydrolyzed to glucose using a mixture of exo-1,3-βglucanase and β-glucosidase. An additional sample portion undergoes hydrolysis of the α-Glucans to glucose using amyloglucosidase plus a-amylase. The glucose in both sample test portions are measured using GOPOD reagent. β-Glucan is determined by difference between the total glucans and α-glucans.

Acceptable matrices

Brewers yeast, yeast extract, dried mushrooms, mushrooms

Unacceptable matrices

Samples containing cereal grain-based Beta Glucan and/or oat-based Beta Glucan

Limit of quantitation

1%

Equipment

Waterbath, Spectrophotometer

Method reference

Megazyme K-YBGL

Reportable unit

% (w/w)

Sample size requirements

10g

*Prices and offerings are subject to change without notice.

Common Beta-Glucan (Mushroom, Yeast) Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

Unfortunately, this test only reports Beta-glucan.

Tinture samples must have enough beta-glucan present to be dected by this method. Please view current LOQ/LODs on the assay's tech data sheet.

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