Technical data sheet
Aliquots of the sample are dispensed into a petri dish containing a non-specific nutrient-rich media (Plate Count Agar) and incubated at 35°C under anaerobic (without oxygen) conditions for 48 hours.
Foods and food ingredients
Cosmetics, non-food samples, water
Limit of quantitation
Solid food <10 CFU/g Liquid <1 CFU/g. The limit of quantitation can vary based on matrix.
Standard Microbiology Equipment
Wadsworth Anaerobic Bacteriology Manual 4th Edition Compendium of Methods for the Microbiological Examination of Foods 4th Edition:Chapter 6
Sample size requirements
Information required by submitter
Please supply estimates
This assay is used to determine the number of anaerobic (growth without oxygen) mesophilic (growth at body temperatures i.e. 35 C) bacteria contained in a food product or ingredient. No information about potential food borne pathogens can be inferred from anaerobic plate counts. Some foods (raw meats, raw commodities) can naturally contain high levels of anaerobic mesophilic bacteria.