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Amylose and Amylopectin Test

  • 271 per analysis
    • |
  • 12 – 15 Business Days
    • |
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Amylose and Amylopectin Test
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Technical data sheet

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Method description

The sample is completely solubilized by DMSO and fats removed by an alcohol precipitation of the starch. Upon further solubilization and dilution of the sample with acetate buffer, the amylopectin is removed by precipitation with a Concanavilin A. The amount of amylose in the sample is quantified by the conversion of glucose with specific enzymes and measured using a GOPOD reaction. A portion of the sample, prior to the addition of Concanavilin A, is quantified in the same manner yielding the amount of total starch.

Amylose is determined by a ratio of amylose to total starch. Amylopectin is determined by difference, where together they equal 100%.

Acceptable matrices

Grains, flours, rice, starches

Unacceptable matrices

Samples low in starch

Limit of quantitation

5% – 95% Amylose

Equipment

Spectrophotometer

Method reference

Megazyme K-AMYL

Reportable unit

Amylose % of Total Starch

Amylopectin % of Total Starch

Sample size requirements

5 grams

Additional information

If you are unable to provide an estimate, a complete sample description is helpful.

*Prices and offerings are subject to change without notice.

Common Amylose and Amylopectin Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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