Amylose and Amylopectin Test
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Price
$285 per analysis
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Turnaround time 12 - 15 Business Days
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Rush Available
Technical data sheet
Print- Method description
The sample is completely solubilized by DMSO and fats removed by an alcohol precipitation of the starch. Upon further solubilization and dilution of the sample with acetate buffer, the amylopectin is removed by precipitation with a Concanavilin A. The amount of amylose in the sample is quantified by the conversion of glucose with specific enzymes and measured using a GOPOD reaction. A portion of the sample, prior to the addition of Concanavilin A, is quantified in the same manner yielding the amount of total starch. Amylose is determined by a ratio of amylose to total starch. Amylopectin is determined by difference, where together they equal 100%.
- Acceptable matrices
Grains, flours, rice, starches
- Unacceptable matrices
Foods low in starch
- Limit of quantitation
5% - 95% Amylose
- Equipment
Spectrophotometer
- Method reference
Megazyme K-AMYL
- Reportable unit
Amylose % of Total Starch Amylopectin % of Total Starch
- Sample size requirements
10 g
- Information required by submitter
Please supply estimates
- Additional information
If you are unable to provide an estimate, a complete sample description is helpful.
Common Amylose and Amylopectin Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
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