Amylose and Amylopectin Test
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271 per analysis | -
12 – 15 Business Days | -
Rush Available
Technical data sheet
PrintMethod description
The sample is completely solubilized by DMSO and fats removed by an alcohol precipitation of the starch. Upon further solubilization and dilution of the sample with acetate buffer, the amylopectin is removed by precipitation with a Concanavilin A. The amount of amylose in the sample is quantified by the conversion of glucose with specific enzymes and measured using a GOPOD reaction. A portion of the sample, prior to the addition of Concanavilin A, is quantified in the same manner yielding the amount of total starch.
Amylose is determined by a ratio of amylose to total starch. Amylopectin is determined by difference, where together they equal 100%.
Acceptable matrices
Grains, flours, rice, starches
Unacceptable matrices
Samples low in starch
Limit of quantitation
5% – 95% Amylose
Equipment
Spectrophotometer
Method reference
Megazyme K-AMYL
Reportable unit
Amylose % of Total Starch
Amylopectin % of Total Starch
Sample size requirements
5 grams
Additional information
If you are unable to provide an estimate, a complete sample description is helpful.
Common Amylose and Amylopectin Test Testing Questions
While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.
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Medallion Labs
A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.
