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Aflatoxin Test

  • 143 per analysis
  • |
  • 15 Business Days
  • |
  • Rush Available
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Technical data sheet


Method description

The sample is extracted into solvent and the extract is added to a solution of enzyme-conjugated toxin. The mixture is then transferred to an antibody-coated plastic well where the free toxin and the enzyme-conjugated toxin compete for antibody sites. After rinsing, a color developing agent and a stop solution are added. The color of the well is evaluated for the presence/quantity of aflatoxin in the sample.

Acceptable matrices

Corn, cornmeal, corn gluten meals, corn/soy blend, wheat rice, milled rice, milo, soy, whole cottonseed, cottonseed meal, raw peanuts, peanut butter, and mixed feeds

Unacceptable matrices

This method is not designed for the analysis of psyllium powder, nutraceuticals, oils, or flavor extracts. Concentrated food additives, colors and flavors may also cause interferences when using ELISA-based test methods.

Limit of quantitation

Range of 5 to 50 ppb


Microwell Strip Reader – Spectrophotometer

Method reference

Neogen 8030

Reportable unit


Sample size requirements

500 grams

Additional information

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.

This test determines the presence of aflatoxins B1, B2, G1 and G2, from the Aspergillus mold, using a direct competitive ELISA (Enzyme-Linked ImmunoSorbent Assays) method in a microwell format. However, the test only provides a Total Aflatoxin result. It does not provide results that are specific to the individual mycotoxin species.

Concentrated food additives, colors and flavors may cause interferences when using ELISA-based test methods.

*Prices and offerings are subject to change without notice.

Common Aflatoxin Test Testing Questions

Aflatoxins are tested using an LCMS/MS instrument. Instead of an overall Aflatoxin value, results for Aflatoxin B1, B2, G1, and G2 are individually reported.

Food manufacturers should be aware of aflatoxin regulations and guidelines since aflatoxins are potent carcinogens that impact human health and safety. The FDA also regulates aflatoxins.

Aflatoxins are not readily tastable or produce any aroma. Sometimes, the presence of visible mold or mold-like smell can be an indicator of aflatoxin contamination, but this would not be directly from aflatoxins.

Aflatoxins are most common in corn, wheat, rice, peanuts, tree nuts, straw, and sesame seeds. There are many foods that can be contaminated with aflatoxins since aflatoxins are produced by fungi species that are found across the globe and the environment.

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.