Technical data sheet
The sample is extracted into solvent and the extract is added to a solution of enzyme-conjugated toxin. The mixture is then transferred to an antibody-coated plastic well where the free toxin and the enzyme-conjugated toxin compete for antibody sites. After rinsing, a color developing agent and a stop solution are added. The color of the well is evaluated for the presence/quantity of aflatoxin in the sample.
Corn, cornmeal, corn gluten meals, corn/soy blend, wheat rice, milled rice, milo, soy, whole cottonseed, cottonseed meal, raw peanuts, peanut butter, and mixed feeds
This method is not designed for the analysis of psyllium powder, nutraceuticals, oils, or flavor extracts. Concentrated food additives, colors and flavors may also cause interferences when using ELISA-based test methods.
Limit of quantitation
Range of 5 to 50 ppb
Microwell Strip Reader – Spectrophotometer
Sample size requirements
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.
This test determines the presence of aflatoxins B1, B2, G1 and G2, from the Aspergillus mold, using a direct competitive ELISA (Enzyme-Linked ImmunoSorbent Assays) method in a microwell format. However, the test only provides a Total Aflatoxin result. It does not provide results that are specific to the individual mycotoxin species.
Concentrated food additives, colors and flavors may cause interferences when using ELISA-based test methods.