Vitamin B12 (Cyanocobalamin) Test
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Price
$160 per analysis
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Turnaround time 7 – 9 Business Days
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Rush Available
Technical data sheet
Print- Method description
This is a microbiological method employing the organism Lactobacillus leichmannii to determine vitamin B12 present in food and vitamin concentrates. Vitamin B12 is extracted from a ground, homogenized sample into a solution of sodium phosphate dibasic, citric acid and sodium bisulfite by autoclaving for 10 minutes at 121 - 123 °C. The pH of the solution is adjusted to 6.0 and the diluted extract is added to a basal medium containing all required growth nutrients for L. leichmannii except vitamin B12. The mixture is inoculated with the microorganism and then incubated for 22 hours at 37°C. The growth response of the microorganism is compared quantitatively to that of known standard solutions of cyanocobalamin (vitamin b12). This method is semi-automated utilizing an automated pipettor system and an automated plate reader system.
- Acceptable matrices
Cereals, vitamin concentrates, fruit juices, nutritional supplements, non-fat dairy powders, and meal replacement beverages containing naturally occurring and/or added vitamin B12.
- Unacceptable matrices
Milk-based infant formula or high-fat high-protein supplements. Premixes/concentrates/supplements above 0.8%
- Limit of quantitation
0.2 µg/100g to 800,000 µg/100g
- Equipment
Automated pipettor system Automated plate reader system
- Method reference
AOAC 952.20 and 986.23
- Reportable unit
µg/100g
- Sample size requirements
10 g
- Information required by submitter
Please supply estimates
- Additional information
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01. This method is not designed for use on vitamin concentrates.
Common Vitamin B12 (Cyanocobalamin) Test Testing Questions
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
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