Price of the test:
$139 per analysis
Acceptable Matrices:
This method is applicable to cereals, fruit juices, nutritional supplements and meal replacement beverages containing naturally occurring and/or added vitamin B12.
Unacceptable Matrices:
This method is not recommended for use on milk-based infant formulas and is not designed for use on vitamin concentrates.
Limit of Quantitation:
0.2 µg/100g to 800,000 µg/100g
Sample Size Requirements:
10 grams
Equipment:
Automated pipettor system
Automated plate reader system
Reportable Units:
µg/100g
Turn Around Time:
7-9 Business Days
Is Rush Available?
Yes
Method Reference:
AOAC 952.20 and 986.23
Method Description:
This is a microbiological method employing the organism Lactobacillus leichmannii to determine vitamin B12 present in food and vitamin concentrates. Vitamin B12 is extracted from a ground, homogenized sample into a solution of sodium phosphate dibasic, citric acid and sodium bisulfite by autoclaving for 10 minutes at 121 – 123 °C. The pH of the solution is adjusted to 6.0 and the diluted extract is added to a basal medium containing all required growth nutrients for L. leichmannii except vitamin B12. The mixture is inoculated with the microorganism and then incubated for 22 hours at 37°C. The growth response of the microorganism is compared quantitatively to that of known standard solutions of cyanocobalamin (vitamin b12). This method is semi-automated utilizing an automated pipettor system and an automated plate reader system.
Information Required by Submitter:
Please supply an estimate with your sample.
Additional Information:
This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.
This method is not designed for use on vitamin concentrates.