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Vitamin B12 (Cyanocobalamin) Test

  • 154 per analysis
  • |
  • 12-14 Business Days
  • |
  • Turnaround Time Delay
  • |
  • Rush Available
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Vitamin B12 (Cyanocobalamin) Test

Technical data sheet


Method description

This is a microbiological method employing the organism Lactobacillus leichmannii to determine vitamin B12 present in food and vitamin concentrates. Vitamin B12 is extracted from a ground, homogenized sample into a solution of sodium phosphate dibasic, citric acid and sodium bisulfite by autoclaving for 10 minutes at 121 – 123 °C. The pH of the solution is adjusted to 6.0 and the diluted extract is added to a basal medium containing all required growth nutrients for L. leichmannii except vitamin B12. The mixture is inoculated with the microorganism and then incubated for 22 hours at 37°C. The growth response of the microorganism is compared quantitatively to that of known standard solutions of cyanocobalamin (vitamin b12). This method is semi-automated utilizing an automated pipettor system and an automated plate reader system.

Acceptable matrices

This method is applicable to cereals, fruit juices, nutritional supplements and meal replacement beverages containing naturally occurring and/or added vitamin B12.

Unacceptable matrices

This method is not recommended for use on milk-based infant formulas and is not designed for use on vitamin concentrates.

Limit of quantitation

0.2 µg/100g to 800,000 µg/100g


Automated pipettor system
Automated plate reader system

Method reference

AOAC 952.20 and 986.23

Reportable unit


Sample size requirements

10 grams

Information required by submitter

Please supply an estimate with your sample.

Additional information

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.

This method is not designed for use on vitamin concentrates.

*Prices and offerings are subject to change without notice.

Common Vitamin B12 (Cyanocobalamin) Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.