Technical data sheet
Samples (defatted if necessary) are digested with three enzymes (alpha-amylase, protease and amyloglucosidase) to remove starch and protein. Ethanol is added to precipitate soluble dietary fiber. The resulting solution is filtered and the residue is dried and weighed. Protein and ash are determined on the residue. Total dietary fiber (TDF) is calculated as the percent residue minus protein and ash.
All foods and food products
Standard Gravimetric Analytical Chemistry Equipment
AOAC 991.43 with modifications
Sample size requirements
Information required by submitter
Please supply expected estimates. Indicate matrix type and indicate if samples are high in fat (>5%). Indicate whether the sample contains fructans (inulin or fructooligosaccharides (FOS)), or psyllium, flax and gum (i.e. guar gum).
This test is ISO/IEC 17025 accredited through A2LA, Certificate #2769.01.
Dietary Fiber is the portion of the carbohydrates which is not digested in the human gut. It is composed of both soluble and insoluble components. These methods are applicable to all food products, however samples containing psyllium or fructans should be submitted under specific test codes. High-fat (>5%) samples require special handling.