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Home / Amino Acid Profile Test - Base Hydrolyzed Amino Acids (Tryptophan) Test

Amino Acid Profile Test - Base Hydrolyzed Amino Acids (Tryptophan) Test

  • Price

    $225 per analysis

  • Turnaround time

    12 - 15 Business Days

  • Rush


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Amino Acid Profile Test - Base Hydrolyzed Amino Acids (Tryptophan) Test

Technical data sheet


Method description

Tryptophan is liberated from proteins by alkaline hydrolysis in 4.2N LiOH at 110°C for 22 hours. Hydrolysis is performed under near-vaccum to protect tryptophan from oxygenm and 5-methyl tryptophan is included as an internal standard to account for any losses during hydrolysis. Sample extracts are then diluted and filtered. Analysis is performed on a reversed-phase ultra-performance liquid chromatograph with fluroescence detection (UPLC-FLR).

Acceptable matrices

Food and Food Ingredients

Limit of quantitation




Method reference

AOAC 988.15

Reportable unit


Sample size requirements

50 g

Information required by submitter

Please supply estimates

Additional information

Results will be reported as "Tryptophan as Dehydrate" to signify that the reported value for the individual amino acid does not include the molecular weight of water. If you are in need of the hydrated tryptophan value (individual amino acid that includes the molecular weight of one water molecule) please let us know at the time of sample submission.

*Prices and offerings are subject to change without notice.

Common Amino Acid Profile Test - Base Hydrolyzed Amino Acids (Tryptophan) Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.