Amino Acid Profile Test - Acid Hydrolyzed Amino Acids Test
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Price
$325 per analysis
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Turnaround time 12 – 15 Business Days
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Rush Available
Technical data sheet
Print- Method description
Samples are digested in 6N HCl for 24 hours at 110°C, liberating amino acids from protein. Cysteine/cystine, methionine, and tryptophan are partially/completely destroyed by this process and are not quantified by this method. Norleucine is used as an internal standard to account for any losses during hydrolysis. Sample extracts are then diluted and filtered. Analysis is performed on a cation-exchange high-performance liquid chromatograph this with post-column ninhydrin (NIN) derivatization and UV/Vis detection.
Included in Test:
Alanine, Arginine, Aspartic Acid, Glutamic Acid, Glycine, Histidine, Isoleucine, Leucine, Lysine, Phenylalanine, Proline, Serine, Threonine, Tyrosine and Valine
- Acceptable matrices
Food and Food Ingredients
- Limit of quantitation
0.0005%
- Equipment
HPLC-UV
- Method reference
AOAC 982.30 with modifications
- Reportable unit
%
- Sample size requirements
20 g
- Information required by submitter
Please supply estimates
- Additional information
Results will be reported as "Hydrolyzed Amino Acids as Dehydrates" to signify that the reported values for the individual amino acids in this screen do not include the molecular weight of water. If you are in need of the hydrated amino acid values (individual amino acids that include the molecular weight of one water molecule) please let us know at the time of sample submission. Note: Cystine, Methionine and Tryptophan are not detected by this method.
Common Amino Acid Profile Test - Acid Hydrolyzed Amino Acids Test Testing Questions
AOAC 982.30 (mod)
Tryptophan, cystine and methionine are run as separate tests, as this method destroys them.
This test is designed to measure overall concentration of specific amino acids. Due to this, there are several limitations to be aware of: this method does not cover all essential amino acids (see our Amino Acid Screen package if this is required), and this test does not provide information on whether the amino acids were pre or post-hydrolyzed, or provide a specific value on total protein content.
Amino acids (as well as Dumas Protein) are used to calculate the Limiting Amino Acid value which in turn is used in calculating a PDCAAS score. This is one measure of protein quality.
We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.
Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.
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