Technical data sheet
Samples are digested in 6N HCl for 24 hours at 110°C, liberating amino acids from protein. Cysteine/cystine, methionine, and tryptophan are partially/completely destroyed by this process and are not quantified by this method. Norleucine is used as an internal standard to account for any losses during hydrolysis. Sample extracts are then diluted and filtered. Analysis is performed on a cation-exchange high-performance liquid chromatograph this with post-column ninhydrin (NIN) derivatization and UV/Vis detection.
Food and Food Ingredients
Limit of quantitation
AOAC 982.30 with modifications
Sample size requirements
Information required by submitter
Please supply estimates
Results will be reported as "Hydrolyzed Amino Acids as Dehydrates" to signify that the reported values for the individual amino acids in this screen do not include the molecular weight of water. If you are in need of the hydrated amino acid values (individual amino acids that include the molecular weight of one water molecule) please let us know at the time of sample submission. Note: Cystine, Methionine and Tryptophan are not detected by this method.