Included in Test:
Alanine, Arginine, Aspartic Acid, Glutamic Acid, Glycine, Histidine, Isoleucine, Leucine, Lysine, Phenylalanine, Proline, Serine, Threonine, Tyrosine and Valine
Price of the test:
$300 per analysis
Acceptable Matrices:
Nearly all raw and processed foods or ingredients.
Limit of Detection:
The detection limit is 0.1%
Sample Size Requirements:
Please provide at least 2g of sample for those products with low protein (<5%). Please provide at least 300mg of sample for those products with high protein (>5%).
Equipment:
HPLC-UV
Reportable Units:
%
Turn Around Time:
15 Business Days
Is Rush Available?
No
Method Reference:
Ion Exchange Chromatography of amino acid profile based on AOAC Official Method 994.12. Internally developed method for sample hydrolysis and extraction.
Method Description:
The peptide linkages in the food sample’s proteins are broken by acid hydrolysis. After the acid is removed, the sample is diluted with a buffer solution and filtered. The sample is then injected on an HPLC. Responses are compared to a known calibration standard.
Information Required by Submitter:
An estimate of the total nitrogen or total protein in the sample must be submitted. Medallion Laboratories cannot offer the appropriate test if this information is not available. Alternatively include a request for protein analysis from Medallion Laboratories when submitting your sample.
Additional Information:
This test measures fifteen amino acids, including 8 of the 9 essential amino acids. The amino acids analyzed include: Alanine, Arginine, Aspartic Acid, Glutamic Acid, Glycine, Histidine, Isoleucine, Leucine, Lysine, Phenylalanine, Proline, Serine, Threonine, Tyrosine, and Valine.
Note: Cysteine, Methionine and Tryptophan are not detected in our analysis.