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Amino Acid Profile Test - Acid Hydrolyzed Amino Acids Test

  • Price

    $325 per analysis

  • Turnaround time

    12 - 15 Business Days

  • Rush


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Amino Acid Profile Test - Acid Hydrolyzed Amino Acids Test

Technical data sheet


Method description

Samples are digested in 6N HCl for 24 hours at 110°C, liberating amino acids from protein. Cysteine/cystine, methionine, and tryptophan are partially/completely destroyed by this process and are not quantified by this method. Norleucine is used as an internal standard to account for any losses during hydrolysis. Sample extracts are then diluted and filtered. Analysis is performed on a cation-exchange high-performance liquid chromatograph this with post-column ninhydrin (NIN) derivatization and UV/Vis detection.

Included in Test:

Alanine, Arginine, Aspartic Acid, Glutamic Acid, Glycine, Histidine, Isoleucine, Leucine, Lysine, Phenylalanine, Proline, Serine, Threonine, Tyrosine and Valine

Acceptable matrices

Food and Food Ingredients

Limit of quantitation




Method reference

AOAC 982.30 with modifications

Reportable unit


Sample size requirements

20 g

Information required by submitter

Please supply estimates

Additional information

Results will be reported as "Hydrolyzed Amino Acids as Dehydrates" to signify that the reported values for the individual amino acids in this screen do not include the molecular weight of water. If you are in need of the hydrated amino acid values (individual amino acids that include the molecular weight of one water molecule) please let us know at the time of sample submission. Note: Cystine, Methionine and Tryptophan are not detected by this method.

*Prices and offerings are subject to change without notice.

Common Amino Acid Profile Test - Acid Hydrolyzed Amino Acids Test Testing Questions

While our sales and customer service team are happy to answer general testing questions, consultations typically start at a rate of $250 per 30 minutes regarding the interpretation of reported test results or questions about our test methods. We cannot consult on regulations, tolerance limits, or claims.

We make every effort to keep our methods and detection limits up to date according to the latest standards and qualifications. If you have any questions regarding the limit of detection/quantification or method references, please contact our Customer Service team at 1-800-245-5615.

Our standard turnaround time is 10 business days for most assays. There are some assays that require a longer turnaround time. We also offer a RUSH service that is half the time of the standard turnaround time of the assay at double the cost of the assay. A few assays that we provide cannot be rushed due to the nature of the test. Please check the specific assay you are interested in regarding the ability to RUSH the turnaround time.

Medallion Labs, a division of General Mills, offers multiple areas of expertise for analytical testing and product evaluation for the food and ingredient industry. Our areas of expertise include Chemical Analysis, Nutritional Analysis, Food Safety, Shelf Life Studies, Microbiology, Specialty Fibers, Physical Testing, Allergen Testing, as well as Product Evaluation (which includes Product Performance and Sensory Evaluation) and Technical Consulting/Method Development. Our methods are developed for the testing of food products and ingredients.

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Medallion Labs

A food testing program designed with mid-market and enterprise food and ingredient manufacturers in mind.